EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7696

Fam185a family with sequence similarity 185, member A ( MGI:2140983)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007456. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007456_01	euxassay_007456_02	euxassay_007456_03	euxassay_007456_04

euxassay_007456_05	euxassay_007456_06	euxassay_007456_07	euxassay_007456_08	euxassay_007456_09

euxassay_007456_10	euxassay_007456_11	euxassay_007456_12	euxassay_007456_13	euxassay_007456_14

euxassay_007456_15	euxassay_007456_16	euxassay_007456_17	euxassay_007456_18	euxassay_007456_19

euxassay_007456_20	euxassay_007456_21	euxassay_007456_22	euxassay_007456_23	euxassay_007456_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7696_wholemount_strong.wlz

7696_wholemount_moderate.wlz

7696_wholemount_weak.wlz

7696_wholemount_possible.wlz

7696_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7696_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8981
Entity Detected:	Fam185a, family with sequence similarity 185, member A ( MGI:2140983)
Sequence:	sense strand is shown >T8981 GCGGAGCCGTGCATGCTGTCCCCTTCGTGACTGACCCGAGGCAGCTCGTGGTTCTTGTGGTCCCGGGACC AGCGAGCCAAGGCTGAAGCGTTCCAAGGGAGAGCATGTTCGGCCGCCTCCCTCCTTGCGCGTCCCGAGTG CGGGCGGGCGCCTTGGTCGGAGCTCTCGGGGCTCGGACTTGCGGCTCCAGTGGGGCGCAGCGGCAGGGAT CAGCGCCCGATGACTCTGGGGCCGGCCTGGCGCGTGGGGCTCTGAGGGAGTGGACGCTGCAGGTGAGCCC GTTTGGGCGACTGAGGGCGCGGCTCCCTTGCCACCTGGCCGTGAGGCCCCTGGATCCCCTTGCGCACCCG GATGGCGACCGCGTGCAGGTGGCGGTGTGCGGCGTGGAGCACGTGGCGCGCGGCCTGGACAGCCTGCAGG TCAAGTATGATGCTGATCGGCAGGAGATGGCCATTCTGTCGGACGACATCGACCCCCAGGCGTCGGTGGA GGTGAACGCGCCCGTCAAATTTGATTTGAGTATTGAGTCATCAGGATCTGGCTCTGTGAAGGTGCAGAAT ATTGAATGTGATAGCTGTAAAATCGACACTGAGCAGGGGACCAGCATCCTGCAGTCTGTGAAGAGTCAAA AGTTGCATGTTCAAACCAAAGGAGGCGATGTGATCTGTTGTGGAACGGTTTACGGAAATATAGATATTCA TGCATCGGATAAAAGTACTGTGAGCATAGAGAAACTTCAGGGAAGTTGTGTTAATATATCTACGGAGGAT GGTTTGCTTCAAGCCAAGTATCTTTATACAGAATCTTCCTTTCTGTCTTCTGCTGCTGGGAATATTGCAC TAGGAAATGTTCATGGAAATATCATCTTACAAAGCAAGATGGGTAACATAACAGTGGATTCGTCCTGTGG ATGTCTAAAAGCCTCCAGTCATCAGGGTGCCATAGATGTGTATGTCAGTCAGCTGGGGGAAGTGGCACTG ACAACAGAAGAAGGTTCTATTGCTGTCAAGGCCCCATCTTCTCTACGAGCTTATTTTAAATTATCAGGCA AAGAGGTAGTTGTGGATGAAGAAGCTCATGTTCAGGAAATGGCCAAAGACTGTAAAGGTGATGGTGTGAC GGTTACAGGACTCATGAATCAAGCAAGCAAACACGAGAGATGGATAAATGCCATTGCCCCCAAAGGCACT GTAAGCTTCCAGCATCAGAGCTGGTTTCAGTCCCTGAAGCTGCCGGACTGAGCGCGGTTTCAGTGCATTG GATTTTGGACGTGGACTAACAAGCAGACGCCAGTGTTCATAGAAACATAAAAAACAACAGCGTTGCCCGT GAGTTCTGGTGAATGGTTTCAGGCGCTTCTGCAAAATGTGTATGTAATTCCGCTATTCATTGTCTGTTGT TCTGTTCCAGATTATAAAAGTCCCCAAGATATGTCTTAGGACCTCCAGTGATACCTGAAACCACAAACAG TATCAACGCTGTACACTGTGGGGTTTTTGCTTTTAATATAAACACACATGACAGCCAAATGCTTTTTCTA TACAACTAAGCAGTTATTTACTCTGGCCAAAGCCCTTACCAGTTTAGTCCCAATATCAAAACTAGATGCC TTTCTTTTTCTTCTTTACAAGTTCACAAACAGTGTGTCCACCCTGACTGTAGGGCCTAGCAGCTTCAGCA GATGATTTTTATTTTTAATTTTTGCTTACTAAGTTGAGAACGTTTACCTTTTTCCTAAAGCACTCTGTGG CTTCTCTTTGCCAGACCTAATTAGTGATGTCAGACATGACACTGTGGTTGTGCTTTAGCGCCATTACTAA GCTAAGGCTTCTTGAACCCTGGTACTGTGTGCTACTGTGACAGTTTGATGGCCTCGGTAACTACTGAGTG ACTAACGGACGGTGAACACCCAGCATGCGCGTACTGGCAAAGGAATGCCTCGCAGCCCAAATGCAATGGC AGATTTCCTCACGCTTCTTCACCGAAGAAATAAAATGCAGAACCTTGGATGGGGGCAAGAGAACAGTGCT ACCCCAGATTTAATAACTTCAGATAGCGTTCCCTCACCGGAGTCTATGTAGATGCTTATGCTGGGGTTCC GCTTAGTGTATCACATGGCCGGGATAAAAGTGTTGTCCAGGCTGAGGATGCTATCTAGGAGCTGCGGGGA GAACTCTGCCAAGAGTTATAGGGAAAATCGGCTTCCTTATACCTGGATGATGTAATTTCTTACTGGCTTC AGCTAATGGTCACCTCGTCTTCTAAAGACAACCACATGCTCACCTTCCTAGTGCAGCCCCTCTTCAGGCA AAACAGAGTCCTTCTCTCATGCCCTGAATGTCTGACTTTCTTATAAGCTGGAACAATCTCTGCTTGGTTG GCCAAGTTATTTAACATGATTATGAGAATATCCTAGGTATGTACACCACTCCCACTAGAAATGGTTATGA AAACGTGGCAGCCATTGGAGTCAGCTCAGGATTCCTGGTATCGGAATTCCCATGGGAAAGACTTCCCTCT TACTATGTCCCTCCTGAGTCATCACTGCCTCCAACTTGTGAATAAACCCTTATGTGGGGAAAGCGATGCT GCCGGGTTTTAGTTGGTCTTCTGTGGGTTAGGATGGACCTCCCTGTGGGAAAGAGCTGCTGTTCTGTCGG CTGACTATGAAAGTGCAGGGCACTTTGACAGCGGTGTGCTTTAGAGAGTCACTTCATGGATTCCATTTCT TTAGAAATGTCAACTGTAACATTAAAAACTATGCTGTATTGAGTAGATCAGTTGGTTGGGTTGTGTATTT TGATTTAGATAAATCAGTGATATTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810783 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810783 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7695 same embryo
	EMAGE:7699 same embryo
	EMAGE:7697 same embryo
	EMAGE:7694 same embryo
	EMAGE:7698 same embryo
	EurExpress:euxassay_007456 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source