EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7697

Ift81 intraflagellar transport 81 homolog (Chlamydomonas) ( MGI:1098597)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007462. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007462_01	euxassay_007462_02	euxassay_007462_03	euxassay_007462_04

euxassay_007462_05	euxassay_007462_06	euxassay_007462_07	euxassay_007462_08	euxassay_007462_09

euxassay_007462_10	euxassay_007462_11	euxassay_007462_12	euxassay_007462_13	euxassay_007462_14

euxassay_007462_15	euxassay_007462_16	euxassay_007462_17	euxassay_007462_18	euxassay_007462_19

euxassay_007462_20	euxassay_007462_21	euxassay_007462_22	euxassay_007462_23	euxassay_007462_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7697_wholemount_strong.wlz

7697_wholemount_moderate.wlz

7697_wholemount_weak.wlz

7697_wholemount_possible.wlz

7697_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7697_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8978
Entity Detected:	Ift81, intraflagellar transport 81 homolog (Chlamydomonas) ( MGI:1098597)
Sequence:	sense strand is shown >T8978 CCGAGGGTGGGGAGGTGGTTTCGTGAGGAGGATTTGAGTTTTAAGCTATAACATTAATCATGAGTGACCA AATCAAATTCATCGTGGACAGCCTCAATAAGGAGCCTTTTAAGAAGAATTATAACTTAATCACATTTGAT TCTTTGGGACCAATGCAACTATTGCAAGTTCTCAATGATGTCCTGGCTGAGATTGACCCCAAGCAAGATG TTGATATCAGAGAGGAGATGCCAGAGCAGACAGCCAAACGAATGTTGAATCTTCTTGGTATCCTTAAGTA CAAACCTCCGGGAAATGCCACAGACATGAGCACCTTCCGTCAGGGCTTGGTGATTGGAAGTAAACCTGTG ATTTACCCAGTGCTCCACTGGCTTCTTCAGCGGAGTAATGAACTAAAGAAAAGGGCATATTTAGCTCGTT TCTTAATAAAACTGGAGGTGCCAAGTGAGTTTCTTCAAGATGAGACCGTGGCTGACACCAATAAACAGTA TGAAGAGCTGATGGAAGCCTTTAAAACTCTGCATAAAGAGTGTGAGCAGCTCAAAACATCTGGATTCTCT ACAGCAGAAATCAGAAGGGATATCAGCGCAATGGAGGAGGAAAAAGACCAGCTCATGAAGAGAGTTGAAC GGCTGAAGAAGAGGGTGGAGACCGTTCAGAACCATCAGCGAATGCTTAAAATAGCAAGACAGCTTCGAGT TGAGAAAGAGAGAGAAGAATTTCTTGCACAACAGAAACAGGAACAAAAGAATCAGCTGTTTCATGCGGTG CAGAGATTGCAGAGAGTGCAAAATCAGCTGAAGAGCATGCGGCACGCAGCAGCGGATGCAAAGCCTGAGA GTTTAATGAAGAGGCTAGAGGAGGAGATAAAATTTAACTCATACATGGTCACTGAAAAATTTCCAAAAGA ATTAGAAAGCAAGAAAAAGGAATTACATTTTTTACAGAAGGTGGTTTCTGAGCCAGCTATGGGCCACTCT GACCTTCTCGAACTTGAGACTAAAGTCAATGAAGTAAACACGGAGATCAATCAGTTGATTGAAAAGAAAA TGATGAGAAACGAGCCAATTGAAGGCAAGCTCTCACTGTACAGGCAACAGGCATCCATCATTTCTCGTAA GAAAGAAGCCAAAGCTGAGGAGCTGCAGGAGACCAAGGAGAAGCTGGCCAGCCTTGAGAGAGAGGTGTTG GTGAAAACAAACCAGACCCGGGAGTTCGACGGCACTGAGGTTCTGAAGGGAGATGAGTTCAAGCGATACG TCAGTAAACTTCGAAGCAAAAGCACAGTTTTCAAAAAGAAGCATCAGATAATAGCTGAATTTAAGGCTGA GTTTGGCCTCCTGCAGAGGACAGAAGAACTTCTGAAGCAGCGTCAGGAAACCATTCAGCATCAACTGCGA ACTATTGAAGAAAAGAAGGGTATATCTGGATACAGTTACACCCAAGAGGAGCTTGAGAGAGTGTCGGCCC TGAAGAGTGAGGTTGACGAGATGAAAGGCCGGACCCTGGATGACATGTCAGAGATGGTGAAAAAACTGAA TTCCTTGGTATCAGAAAAGAAGTCAGCTTTAGCCCCAGTTATAAAAGAACTACGGCAGTTGCGTCAGAAA TGTCAAGAACTAACCCAGGAGTGTGATGAGAAGAAAGCCCAGTATGATAGCTGTGCAGCAGGCCTTGAAA GCAACCGGTCCAAATTAGAGCAGGAAGTGCGAGGACTTCGTGAGGAATGTCTTCAAGAAGAAAGTAAATA CCACTATACAAACTGCATGATTAAGAACCTAGAAGTTGAGCTTCGTCGAGCGACTGATGAGATGAAGGCG TACGTCTCTTCTGATCAGCAAGAGAAGAGGAAAGCAATTAGGGAACAGTATACCAAAAATATTACTGAGC AAGAAAACCTTGGTAAGAAACTCCGGGAAAAACAAAAAGCTGTACGAGAAAGTCATGGTCCAAACATGAA GCAAGCAAAAATGTGGCGTGATCTTGAACAACTGATGGAATGCAAAAAACAGTGCTTTCTAAAGCAGCAA AGCCCAGCTTCCATCGGCCAGGTAATTCAAGAGGGCGGAGAGGACCGGCTGGTTCTCTGAGCCCTGTGTC GGCCGGGCTTAAGTCGCATCTGCGTAACGTGATTTCTTCTCGGAAGTGACTTATTCATCCTTTGCTTTCA GACTAGCCGTCCCTTCTCAGTTGTCTTGGAAATGAAGTGAAGGCAGATGAGGTTTTAAAGGTCTTTTTAA CTGCAGGAGAGGCTTTCGTTGTTTGTTTAGCTTAGCCGTCTAGTGGTGTCTCCTGAGTTTTATGGGAAAG GGCTAAGTTTGCTCTCTGTAATGTAAGCATGTGTCCATTAAGAACCACGTAGCTTCTCAAGCTGTGAGGA CTGACGGGTAATGCTCTTCTCAGTCTGTCTCTGACTTGGAGGACTCTTTTAGGAACTAGGAGTTCGTTTG AAGAAGCTTTTGTCTGAAGTCAGCAGTCGCTACAGTTTCACTTCAGTTTCTTTCTCTTCTATAAAATAAA ACCAGTTAATGTTGTGAAAAGCAATTGTGATGTGATGTGATTGCAAAGTAAATAAAGCCTGCGGTGGCCT GCCGTAGCTGTTGATCTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810406 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810406 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7696 same embryo
	EMAGE:7695 same embryo
	EMAGE:7699 same embryo
	EMAGE:7694 same embryo
	EMAGE:7698 same embryo
	EurExpress:euxassay_007462 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source