EMAGE mouse gene expression spatial database

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EMAGE:7699

Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 ( MGI:2687284)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007461. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007461_01	euxassay_007461_02	euxassay_007461_03	euxassay_007461_04

euxassay_007461_05	euxassay_007461_06	euxassay_007461_07	euxassay_007461_08	euxassay_007461_09

euxassay_007461_10	euxassay_007461_11	euxassay_007461_12	euxassay_007461_13	euxassay_007461_14

euxassay_007461_15	euxassay_007461_16	euxassay_007461_17	euxassay_007461_18	euxassay_007461_19

euxassay_007461_20	euxassay_007461_21	euxassay_007461_22	euxassay_007461_23	euxassay_007461_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7699_wholemount_strong.wlz

7699_wholemount_moderate.wlz

7699_wholemount_weak.wlz

7699_wholemount_possible.wlz

7699_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7699_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8982
Entity Detected:	Pfkfb4, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 ( MGI:2687284)
Sequence:	sense strand is shown >T8982 AGAGTTGGAGCCGCTAGAGGGTGGGCGGGGCCGGAGGGGCGGGACCTCGGGTGGCCCGAGGTGGCCCGGT TGCTGGGTGAGTAGCAGCGCAGTCCGATTCATCCCAGCCCAGGGATGGCGTCTCCACGGGAACTGACCCA GAATCCCCTGAAGAAGATCTGGATGCCGTACAGCAATGGCAGGCCCGCTCTGCACGCCAGCCAGCGTGGT GCCCGGGGCAAGACCTACATTTCTAAGAAGCTGACGCGGTACCTCAACTGGATTGGCGTGCCTACTCGGG AATTCAACGTTGGTCAGTATCGCCGGGACATAGTCAAGACGTACAAATCTTTTGAGTTTTTCCTCCCAGA CAATGAAGAAGGCCTGAAAATCAGGAAGCAGTGTGCCTTAGCAGCCCTCAGTGATGTCCGGAAGTTCCTC AGTGAGGAGGGGGGACATGTGGCGGTTTTTGATGCAACCAATACGACCCGAGAGCGGAGAGCGATGATCT TTAACTTTGGAGAACAGAATGGCTACAAGACCTTCTTTGTTGAATCTATCTGTGTGGATCCTGAGGTTGT TGCTGCCAACATCGTGCAAGTGAAGCTGGGCAGCCCTGACTATGTCAACCGGGACAGCGACGAAGCCACC GAAGACTTCATGAGGCGCATTGAATGCTATGAAAACTCATATGAGTCGCTGGATGAAGACCTGGACAGGG ATCTGTCCTACATCAAGATCATGGACGTGGGCCAGAGCTATGTGGTGAACCGTGTTGCTGATCACATCCA GAGTCGCATCGTTTATTACCTCATGAACATCCATGTGACACCCCGCTCCATCTACCTCTGCCGGCATGGG GAGAGCGAGCTAAACCTCAAGGGCCGGATTGGTGGGGATCCTGGACTGTCCCCCCGGGGCCGGGAGTTTT CCAAGCATCTGGCCCAGTTCATCAGTGACCAGAACATTAAGGATCTGAAGGTCTGGACGAGCCAGATGAA GAGGACGATCCAGACAGCCGAGGCGCTGAGCGTCCCTTATGAGCAGTGGAAGGTCCTCAACGAGATCGAT GCGGGCGTCTGTGAGGAAATGACCTACGAAGAAATCCAGGATCACTACCCGCTGGAGTTTGCCCTGCGGG ATCAGGACAAGTACCGGTACCGGTACCCGAAGGGTGAGTCCTATGAAGACCTGGTGCAGCGGCTGGAGCC CGTCATCATGGAACTGGAGAGGCAGGAGAATGTGTTGGTCATTTGCCACCAGGCTGTCATGCGCTGCCTC CTGGCCTACTTCCTTGACAAGGCAGCTGAAGAGCTGCCCTACCTCAAATGCCCCTTGCACACAGTCCTGA AGCTCACACCCGTGGCTTACGGTTGTAAAGTGGAGTCCATATTCCTGAACGTGGCAGCTGTGAACACTCA CCGAGACAGACCTCAGAATGTAGACATATCCAGGCCTTCAGAGGAAGCCCTTGTCACAGTCCCTGCTCAC CAGTGACCCTGACCTCTGTCAGAGGGGTCACTCCAGGCCATCTTGTGTTTGCTGTACTGATGGTGCCACC ATGCAGGAACACTCCTGAAGCCATGGGTGGCTAGCAACACTCAGAACCTTCAGCCTGGCCCACCGGCCTC TTTGTTGACCACCAGTGACAGGTTGGGTATGGCTGCTGAGAATCCCTGGGCTATGTGCTCTGCTGCCAGC CCCTCTGCTACAGCACTCGAGCAGTCCACTCACCAGGCTGGCTGCTTCATGACGTTTGCAACCTTAAATG AGACACTGAGACAAAAAACTCTCACTAAGACATTCCACTGTGGCCCTGCTGCCTGATGGGGGCCAGGCAA CCCCTGGAAGAGTTCTACTGCCCTCTCCATAGCTACTCTTGGAGCCAGAGGCTTGATCTTCATGGGGCCT TATGTGGACACCACCCCAGGTGAAGTGGAAAGCCCTTCCTTCCCTGCCTGGAACAGAAGATGGAGCAGGG AGCTTCTACCCACATCTAACCTCTGCATCTGTGGGTACTCAGGAGAGTAAGTGTGGAAAGGACCATGCGT GGCGCCCATTCCTAACGTCTACCAAGTTATACACTGGAATACAGGAGAGCTGCGGCCCAGGCTGCCTCGT CCTAAATTCTCTTTCGGGGGATCCTGTAAAGGCAGGGCAGCTTTAGGAAGTACCTTCTTGGTGCACACAC CAAAGTGCTTATGGAAACTGGTCTCCCCTACCTGGCCGTTGCTGTCACTGTCCACACAGAACTGTACACT GGTAACGGGGACATGCCTTTGTCATGAGAGGCTCAGAACTGGTGGTCCTGTCGGACTGTCTTCCCATTTG CTGGCCTCTACCTGTAGGACTGAGGAAGGAAAGGACAAGGCATGAGGCAGCCCAGACCCCAGGAGGAGTC GCTGGCCCCACTCTAGTTTTGATTCAAGTACAAATTGAGTCCTAGTGCAAACAGCCTTATGAAGCCTGGT GCCTCCCATGCCCCGAGTTCTTGTGGGTCCACATAGCAGAGTCAGGCTCCATGTTTCAGCTGACCAGCAA GGTGATAGGCAGGTTGTGGAGCAGGGCAAGAAACTTTCTACAGCAGTAACTGGTTGCTCTAGTGTCCACA GAGCAGCCATGGGGAAGCCATGCCCTGCATCCAAGAACCGTTCAAAGCCCAAATCTGGCAGAATAGTCCA CCTTTTCCAGCCCTGCCAGTCCTGGGCTGTGTAGCTTGCAGCTGCCATCTGCCCCTGAGAAGTCAGTGCC ATTATTCCAAACTGCTGTCTGTGCGGGCCTAGGGCGGGACTCCTGGGCTCATCTCCTCAGACCTCCATGT CCTAGCTCGGAGCTGGAAGACCATACCACTGCCTTCGGGACATGGGCCCTGCAGCTTCTGCCCCGTGTCC CTCAGAGTCTGAGTGACGTTCATCATATCATCTGGACACTTGTTAGCCATAGCAGATGCCTGTGGTCGGC CACTGTGGATAATGGCTGGATGCAGCTTGAGGCAAAGACCACCAAAGCCTTTTTCCTCTGTGCCCCGGGA AAGCAGGAACTGATGGACCCAGAGGTGTAAGAGCCCAGGGTGGGGGCAGAGGGATGTCAGTGGAGCAGGA GTTGTGCTGGAACAGCCCCTCCCTCCAGCCCCTGAAGTGGCTTCCGCAAGGCAGTCGCATGGGGGGCTGG TTGTCAGGCTTTAGCTGAGTTGGTTCAGGGGTTCACAGGCGGACTGCCTACATTTCCTGCTTTCCCTAGA ATGTATTATCCTGGTTTTCACTTGTGCATTGCACTGTGTCCAGCATTCTTTGCAATAAATACTTTAAAAA ACAAAACAAACAAACAAAAACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810667 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810667 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7696 same embryo
	EMAGE:7695 same embryo
	EMAGE:7697 same embryo
	EMAGE:7694 same embryo
	EMAGE:7698 same embryo
	EurExpress:euxassay_007461 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source