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EMAGE:772

Hoxb1 homeobox B1 ( MGI:96182)
TS11 (7.75 dpc)
in situ hybridisation

Data Images
EMAGE:772
Figure 6B of Wassarman et al., 1997 [PMID:9247335] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The anterior border of the Hoxb1 expression domain is designated by the bottom arrow.
Expression Pattern Description
Spatial Annotation:
EMAGE:772EMAGE:772Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
772_voxel_strong_3D_1.wlz
772_voxel_moderate_3D_1.wlz
772_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:772_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
neural ectoderm
detected detected
regionalThe anterior boundary of expression marks the anterior end of rhombomere 4. The upper arrow indicates the posterior border of Otx2 expression. The lower arrow indicates the anterior border of Hoxb1 expression.
embryo
detected detected
regionalExpression is in all three germ layers.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1331399
Entity Detected:Hoxb1, homeobox B1 ( MGI:96182)
Sequence:sense strand is shown

>MGI:1331399
AGGCCCCCCAGGTTGCCCAAAGGAAGCCGCTGGAGATGCCTCTGACCAGTCCGCGTGCACCTCCCCAGAA
GCCTCGCCCAGTTCCATCACCTCTTGAATTGAACTTCCTAAGTAACTGGGCTTCCAACGCTTGACCAGTT
CTCTCGAAGACTTTCCCAAACTTCACAGCCTTGGTGACCCTCCTCAAGGCCGAGGCACCAGTTTAGAGCT
GCCCAGGAAACTGGGCAGGAGTTGGGAAATGTATTTTTCTCACTCTCAGATCTAGGGGTGGAGGGATGAT
TGATGGCTGGGGATCCTACAGGTCTTGGGACCTGGGGAACACTCAACTCATCAGAGGTCGAAGGAAGGCC
TTTTGGCTTTGATCTGGAGTCAGCCCATCCTTTCGGGCTTCTCCTTTCCCTTCCAACTCAGTTCAGTGCC
TTTGAGCTTAGAGAGTTCTTCTTTCGAATTCTTAGCCCAACTCTTCTTTCTAGGTCTGGGATGTCTAGGC
TGGGAGTCCTTGGGGTTTTTCTGGGGGAGGATGCGTTTGAGTGTGAGCCCAATCGCCACCAACCCTTAAT
CTTAATCCAGCCAACTCACCGCAGACCTTACAATAGCAGACCTTACAATAGCACCGACTGCCTTTGACAT
ACC
nt 868 - nt 1500 of NM_008266.3
Notes:The Hoxb1 (Hox-2.9) probe used in this study by Wassarman et al., 1997 [PMID:9247335] was originally described in Frohman et al., 1990 [PMID:1983472] as a "633 bp Pst1-SalI fragment" (see Figure 3 therein). The SalI restriction site is located in the polylinker at the 3' end.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:7.75 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:paraffin
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Wassarman et al., 1997 [PMID:9247335] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9247335] Wassarman KM, Lewandoski M, Campbell K, Joyner AL, Rubenstein JL, Martinez S, Martin GR 1997 Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development (124):2923-34
 [ PMID:1983472] Frohman MA, Boyle M, Martin GR 1990 Isolation of the mouse Hox-2.9 gene; analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm. Development (110):589-607
Links:MGI:1331430 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI