EMAGE mouse gene expression spatial database

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EMAGE:7722

Sh2b2 SH2B adaptor protein 2 ( MGI:1345171)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007457. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007457_01	euxassay_007457_02	euxassay_007457_03	euxassay_007457_04

euxassay_007457_05	euxassay_007457_06	euxassay_007457_07	euxassay_007457_08	euxassay_007457_09

euxassay_007457_10	euxassay_007457_11	euxassay_007457_12	euxassay_007457_13	euxassay_007457_14

euxassay_007457_15	euxassay_007457_16	euxassay_007457_17	euxassay_007457_18	euxassay_007457_19

euxassay_007457_20	euxassay_007457_21	euxassay_007457_22	euxassay_007457_23	euxassay_007457_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7722_wholemount_strong.wlz

7722_wholemount_moderate.wlz

7722_wholemount_weak.wlz

7722_wholemount_possible.wlz

7722_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7722_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9019
Entity Detected:	Sh2b2, SH2B adaptor protein 2 ( MGI:1345171)
Sequence:	sense strand is shown >T9019 GCGCGCGGCGCGCGAGTCTGGGATCGGAGCGCGCGGAGCTCGGGAGCCTGAAGAGGGAGAGAACCTCAAG GGGGCCGCACCAGGACCGCCAAGACCGCCTCGCAAGCTGCAGGCCAGGGAAGCGGGAGTCACCCCCGCTG CCGCCGCCACCGGCCACCAAGGGCGGAGCTACGGCCCGGAGGGCCATGCGCCACCGGGTCCGGGGATCCC AGCCGCGGTCCGGGCAGCGCTCCTCCGGCGCCCCAGCCCGCGCGGCCGAAGCTGCGCGCCTGTAGCCCGA CGCTGCCATGGGACCAAAGCCATGAATGGTGCCACCCCCAGCTCGGCCGCCGCCCCCGCCCCAGTCCCCG ACTGGCGGCAATTCTGCGAGCTGCACGCGCAGGTGGCTGCTGTGGACTTCGCACACAAGTTCTGCCGCTT CCTTCGGGACAACCCGACCTACGACACGCCTGACGCTGGGACCTCATTCTCCCGCCACTTTGCAGCCAAC TTCTTGGCTGTCTTCAGTGAGGAGGTGCGCCGCGTGCTGGGGTCAGCAGCCGACACTATGGAACCCGAGC CTGCGGTGACCTCGGTGACATCTGCGCTCAAGACTGCAACCTATGGCCACTCCCGCAGCTCCGAGGATGT GTCTGCCCACGCGGCTACCAAGGCGCGTGTCCGAAAGGGATTCTCGCTGCGTAACATGAGCCTGTGTGTG GTGGATGGAGTGCGCGACCTGTGGCACCGGCGCTCGTCCCCAGAGCCCGATGGTGGGGCCACCCCCAAGG CCGCCGAGCCTGCGTCGGAGCCTCGTGACAAATGGACGCGACGCCTGCGGCTAGCACGAACCCTGGCAGC CAAAGTGGAGTTAGTGGACATCCAGCGTGAGGGCGCCCTGCGCTTCATGGTGGCAGACGACGCGGCCTCA GGGCCTGGAGGCACTGCGCAGTGGCAGAAGTGTCGCCTGCTCCTGCGGAGGGCTGTGGCTGGGGAACGTT TCCGCCTGGAGTTCTTCGTGCCACCCAAGGCTTCCAGACCCAAGGTCAGCATCCCCCTGTCGGCCATCAT TGAGGTCCGAACCACTATGCCCCTGGAAATGCCTGAAAAGGACAACACATTTGTGCTCAAGGTGGAGAAC GGAGCAGAATACATCCTGGAAACGATAGACTCACTGCAGAAGCACTCGTGGGTGGCTGACATCCAGGGCT GTGTGGATCCTGGGGACAGTGAGGAAGACACAGGACTGTCTTGTGCACGGGGAGGCTGCCTGGCCAGCCG CGTGGCCTCCTGCAGCTGTGAGCTCCTGACAGATGCAGACATGCCCCGGCCCCCCGAGACGACGACAGCG GTGGGTGCTGTGGTCACAGCTCCACATGGCCGAGCTCGTGACACCGTGGGAGAGTCCCTGGCCCACGTTC CCCTGGAGACTTTCCTGCAAACCCTGGAGTCTTCAGGTGGTGTCAGTGAGAACAATAACCCAGGGGACGA AGGAGCTGAGCTGGACACCGATGCTGAGGCTGAGCTGGAACTTTCCGACTACCCCTGGTTCCATGGGACA TTGTCCCGGGTGAAGGCGGCCCAGCTTGTGCTGGCCGGTGGCCCTCGGAGCCATGGCCTCTTTGTGATCC GCCAAAGTGAGACTAGGCCTGGGGAGTGTGTGCTGACCTTCAACTTCCAGGGCAAGGCCAAGCACCTCCG CCTTTCTCTGAACGGTCACGGCCAGTGTCATGTCCAGCACCTGTGGTTCCAGTCCGTGTTTGATATGCTC CGTCACTTCCACACCCACCCCATCCCCCTGGAGTCAGGAGGATCGGCTGATATCACCCTAAGAAGCTATG TGCGTGCCCAGGGCCCGCCTCCTGATCCAGGACCAGCGCCCAACACTGCGGCCCCAGTTCCAGCCTGCTG GACGGAGCCGGCTGGCCAGCACTACTTCTCCAGCCTGGCCACGGCCACTTGCCCGCCCGCTTCACCGTCC AATGGCGCTGGAGCTTCGTCTTCATCCGGCTCATCGTCCTCTGCCACATCCCTGCCCCCACGCCCAGCCG AGGGGCCGCTCAGTGCGCACAGCCGCAGCAACAGCACAGAGCACCTGCTGGATGCTGCATCTGGAGCCAC TGAGGAGCCCACAGAGGCCACGCTGGGCCGTGCTCGTGCTGTGGAGAATCAGTACTCCTTTTACTAGCTG CCATGCCTTTTACCCACGGGTGTCACGCACATCACAGCAGGCCAAGGACACCTGTTGTCTGTCCTGCCCA CCCACAGCCTGGCTAGCCCACGATGGTCAGCAACACCTAATGAATGGCTAAATACACCTACCTATGGTCT AGCATGGCCAAAATAGCTGGCCAGCCTAGCCACTTCAGACCCCAGGGGCCCACTGTGACCATGTGTGGCA GGCCAAGGCTATATCCACAGTCGATCAAACATCATGATAGCCCGCCAATGATTTCAGGCCCCACCCACTG GTGGATGGCCACTCCCATTAGCAGCTATTTTTATAAGGGCCACCGAATCCATAACAGGCAGCTAGCTCAC TGACAACTAAACACATTTGGTGACTTCAGGCCCTGGCCACTGATAGTTGGTTACTCCTACAGTGGCATGG CCTACCCACTGGCTGCCTGCTTCACTCAAGGGCAGCAAGCCACGTCTATCAAGCCCATGAAGGCCTACCC ACTGATTCCTAGCAACCTCAGGCCCTGCCCACCAGTGTTGGGTCACATACTCCAATAACCTGACCAGGCT GTGGTCTGCCTAGCCTAGGACAGCCTGCCACGCCCACCAGCCCCAGCCCAATGCTGGGGGGAAAAGCCAA GCTCTTCAGTGAAGACAAACATTATTAAAGAGCCTGTTTTAGGGACTAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811850 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811850 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7723 same embryo
	EMAGE:7725 same embryo
	EMAGE:7724 same embryo
	EMAGE:7721 same embryo
	EMAGE:7720 same embryo
	EurExpress:euxassay_007457 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source