EMAGE mouse gene expression spatial database

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EMAGE:7724

Xpc xeroderma pigmentosum, complementation group C ( MGI:103557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007481. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007481_01	euxassay_007481_02	euxassay_007481_03	euxassay_007481_04

euxassay_007481_05	euxassay_007481_06	euxassay_007481_07	euxassay_007481_08	euxassay_007481_09

euxassay_007481_10	euxassay_007481_11	euxassay_007481_12	euxassay_007481_13	euxassay_007481_14

euxassay_007481_15	euxassay_007481_16	euxassay_007481_17	euxassay_007481_18	euxassay_007481_19

euxassay_007481_20	euxassay_007481_21	euxassay_007481_22	euxassay_007481_23	euxassay_007481_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7724_wholemount_strong.wlz

7724_wholemount_moderate.wlz

7724_wholemount_weak.wlz

7724_wholemount_possible.wlz

7724_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7724_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9023
Entity Detected:	Xpc, xeroderma pigmentosum, complementation group C ( MGI:103557)
Sequence:	sense strand is shown >T9023 GGAGACCTGCGGCGTCCTGCGCGGTAGTCCCGAGGAACGCCTCTGGCTAGCATGGCCCCAAAGCGCACCG CAGACGGAAGGCGGCGGAAGCGGGGCCAGAAAACCGAGGACAACAAAGTAGCCCGGCACGAGGAGAGCGT TGCGGATGACTTTGAAGATGAGAAGCAGAAACCCCGAAGGAAGAGCTCTTTCCCGAAAGTCTCTCAAGGA AAGAGGAAGAGGGGCTGCAGTGATCCAGGGGACCCCACAAATGGTGCAGCAAAAAAGAAAGTGGCCAAAG CCACTGCTAAATCCAAGAATCTCAAGGTTCTGAAGGAGGAAGCACTCAGCGACGGGGATGACTTCCGGGA CTCACCAGCTGACTGCAAGAAGGCAAAGAAACACCCAAAAAGCAAGGTGGTGGACCAAGGCACTGATGAA GATGACAGTGAGGATGACTGGGAGGAGGTGGAAGAGCTTACTGAACCTGTGCTGGACATGGGAGAAAATT CTGCCACCTCACCGTCTGACATGCCTGTGAAGGCGGTGGAGATTGAAATTGAAACACCACAGCAGGCGAA AGAACGGGAAAGAAGTGAGAAGATAAAGATGGAGTTTGAGACATACCTGCGGAGGATGATGAAGCGTTTC AATAAAGAAGTACAGGAGAACATGCACAAGGTTCACCTGCTGTGCCTGCTGGCCAGTGGCTTCTATCGAA ATAGCATCTGCCGACAGCCAGATCTGCTGGCCATTGGCCTCTCCATCATTCCAATTCGCTTTACCAAGGT GCCACTTCAAGATAGGGATGCCTACTACCTTTCAAACCTGGTAAAGTGGTTCATCGGAACCTTCACTGTC AACGCTGACCTTTCAGCCAGCGAGCAGGACGACCTGCAGACCACCTTGGAAAGGAGGATTGCCATTTACT CTGCGAGGGATAATGAAGAGTTGGTCCATATATTTCTTCTGATTCTTCGGGCTCTGCAGCTGCTCACCCG GCTGGTCTTGTCTCTGCAGCCCATTCCACTGAAGTCAGCTGTGACAAAGGGGAGGAAATCTTCCAAGGAG ACATCTGTAGAGGGTCCTGGAGGTTCTTCAGAACTCTCTAGTAACAGTCCAGAAAGCCACAACAAACCTA CGACCAGCAGGAGAATCAAAGAAGAAGAAACCTTGTCTGAGGGCAGAGGCAAAGCAACCGCCAGGGGGAA GAGAGGCACAGGCACTGCGGGCAGCAGGCAGCGGAGGAAGCCCTCTTGCAGCGAGGGAGAGGAGGCCGAG CAGAAAGTCCAGGGCCGTCCACATGCCCGGAAGCGGCGTGTGGCTGCCAAGGTGTCATACAAAGAGGAGA GTGAGAGCGATGGGGCAGGCAGCGGCTCTGACTTTGAACCGTCCAGTGGGGAGGGCCAGCATTCCTCTGA TGAGGATTGTGAGCCTGGCCCTCGCAAGCAGAAGAGGGCCTCAGCTCCTCAGAGGACAAAGGCTGGGTCT AAGAGTGCTTCCAAGACCCAACGCGGAAGCCAGTGTGACCCGTCAAGCTTTCCGGAGGCGTCTTCAAGCT CTTCAGGCTGTAAGAGAGGCAAGAAGGTTTCCAGTGGTGCTGAAGAGATGGCAGATAGGAAACCTGCTGG TGTAGACCAGTGGCTGGAGGTGTACTGTGAGCCACAGGCAAAGTGGGTGTGTGTGGACTGTGTACATGGT GTGGTGGGCCAGCCTGTGGCCTGTTACAAATATGCCACCAAACCCATGACCTATGTTGTAGGCATTGACA GTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGACCCAGCCTGGATGACCGCAACCCGCAAGTGCCG GGTTGATGCTGAGTGGTGGGCTGAGACCTTGAGACCCTATCGGAGCCTACTTACGGAGAGGGAGAAGAAG GAAGACCAGGAGTTTCAGGCGAAGCACCTGGACCAGCCTTTGCCCACCTCCATCAGCACATACAAGAACC ACCCTCTGTATGCCCTGAAGCGCCACCTCTTGAAATTCCAGGCTATCTACCCTGAGACAGCTGCGGTGCT CGGGTATTGTCGTGGAGAAGCAGTCTATTCCAGGGATTGCGTGCATACCTTGCACTCCAGGGACACATGG CTGAAGCAAGCAAGAGTGGTGCGGCTTGGAGAAGTGCCCTATAAGATGGTGAAAGGCTTCTCTAACCGTG CCCGGAAAGCCCGGCTCTCGGAGCCCCAGCTGCATGACCACAACGACTTGGGCCTCTATGGTCACTGGCA GACGGAAGAGTATCAGCCTCCTATAGCTGTCGATGGGAAGGTGCCTCGGAATGAGTTTGGGAACGTGTAC CTCTTCCTGCCCAGCATGATGCCCGTCGGCTGTGTCCAGATGACCCTGCCTAACCTGAACCGGGTGGCAC GCAAGCTGGGCATCGACTGCGTGCAGGCCATCACTGGCTTCGATTTCCATGGAGGCTATTGCCATCCAGT AACTGATGGCTACATTGTCTGTGAGGAATTCAGAGACGTGCTGCTGGCTGCTTGGGAGAATGAACAGGCC ATCATTGAAAAGAAGGAGAAGGAGAAAAAGGAAAAGCGGGCGCTGGGGAACTGGAAGCTGCTGGTCATAG GACTCCTCATCAGAGAGAGGCTGAAACTCCGCTATGGGGCCAAGAGCGAGGCAGCAGCTCCCCATGCTGC TGGAGGCGGACTCTCTTCTGATGAAGAGGAAGGGACCAGTTCACAAGCAGAAGCAGCCAGAGTCCTGGCT GCCTCCTGGCCACAGAACCGAGAGGATCCAGAACAGAAGTCCGAGTACACTAAGATGACTCGGAAGAGGC GGGCGGCAGAGGCTTCCCACCTCTTTCCGTTTGAGAAGCTGTGAAGCCAGTGTCCTCCAGCCAGAGAAGG CAGACAGCAAGCAGATGGGCTGAGGACTACAGGCCAGATGGGAGCTGTGCAGCAGATCAGTCTGACACCA ACCCAGCCGGATCCCCATAGGCTGGTGCTGGTGAGCTGCTGTTTGGACCACTTTCCTGGGAGATACCTTC GGATCCCTGGACTTTGGTATCTTTAGAAATGTGAGTTTGTTTTAGAGGGTCTTATTTGTTACCCCAGCTG GCTTCAAATTCAAAATCGCCCTGACTTATACTGAGGGCTGAGATTACAGGCGTATACCACAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6812198 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6812198 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7723 same embryo
	EMAGE:7722 same embryo
	EMAGE:7725 same embryo
	EMAGE:7721 same embryo
	EMAGE:7720 same embryo
	EurExpress:euxassay_007481 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source