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EMAGE:775

Hoxb1 homeobox B1 ( MGI:96182)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:775 EMAGE:775
Figure 9F of Frohman et al., 1990 [PMID:1983472] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 9E of Frohman et al., 1990 [PMID:1983472] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Abbreviations used: 3rd pp, third pharyngeal pouch; BA, branchial arch unit region; lpm, lateral plate mesoderm; ph, pharynx; se, surface ectoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:775EMAGE:775Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
775_voxel_strong_3D_1.wlz
775_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:775_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
not detected not detected
pharynx
strong strong
regionalTranscripts are present in abundance in the surrounding walls of the pharynx, posterior to the developing thyroid, but anterior to the lung bud.
branchial arch
detected detected
regionalHoxb1 transcripts are observed in the dorsal aspect of the second branchial arch unit, are no longer detected in the ventral aspect of the third branchial arch unit, and are now observed in the fourth.
3rd branchial pouch
strong strong
regionalTranscripts are present in abundance in the caudal half of the third branchial cleft (surface ectoderm) and in the caudal half of the third pharyngeal pouch.
3rd branchial groove ectoderm
strong strong
regionalTranscripts are present in abundance in the caudal half of the third branchial cleft (surface ectoderm) and in the caudal half of the third pharyngeal pouch.
mesenchyme
weak weak
regionalExpression is also detected at lower levels in the lateral plate mesoderm (gut-associated mesoderm) near the third pouch.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1331399
Entity Detected:Hoxb1, homeobox B1 ( MGI:96182)
Sequence:sense strand is shown

>MGI:1331399
AGGCCCCCCAGGTTGCCCAAAGGAAGCCGCTGGAGATGCCTCTGACCAGTCCGCGTGCACCTCCCCAGAA
GCCTCGCCCAGTTCCATCACCTCTTGAATTGAACTTCCTAAGTAACTGGGCTTCCAACGCTTGACCAGTT
CTCTCGAAGACTTTCCCAAACTTCACAGCCTTGGTGACCCTCCTCAAGGCCGAGGCACCAGTTTAGAGCT
GCCCAGGAAACTGGGCAGGAGTTGGGAAATGTATTTTTCTCACTCTCAGATCTAGGGGTGGAGGGATGAT
TGATGGCTGGGGATCCTACAGGTCTTGGGACCTGGGGAACACTCAACTCATCAGAGGTCGAAGGAAGGCC
TTTTGGCTTTGATCTGGAGTCAGCCCATCCTTTCGGGCTTCTCCTTTCCCTTCCAACTCAGTTCAGTGCC
TTTGAGCTTAGAGAGTTCTTCTTTCGAATTCTTAGCCCAACTCTTCTTTCTAGGTCTGGGATGTCTAGGC
TGGGAGTCCTTGGGGTTTTTCTGGGGGAGGATGCGTTTGAGTGTGAGCCCAATCGCCACCAACCCTTAAT
CTTAATCCAGCCAACTCACCGCAGACCTTACAATAGCAGACCTTACAATAGCACCGACTGCCTTTGACAT
ACC
nt 868 - nt 1500 of NM_008266.3
Notes:The probe used in this study by Frohman et al., 1990 [PMID:1983472] is a "633 bp Pst1-SalI fragment" (see Figure 3 therein). The SalI restriction site is located in the polylinker at the 3' end.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Frohman et al., 1990 [PMID:1983472] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1983472] Frohman MA, Boyle M, Martin GR 1990 Isolation of the mouse Hox-2.9 gene; analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm. Development (110):589-607
Links:MGI:1340628 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI