EMAGE mouse gene expression spatial database

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EMAGE:7754

Cntrob centrobin, centrosomal BRCA2 interacting protein ( MGI:2443290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007488. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007488_01	euxassay_007488_02	euxassay_007488_03	euxassay_007488_04

euxassay_007488_05	euxassay_007488_06	euxassay_007488_07	euxassay_007488_08	euxassay_007488_09

euxassay_007488_10	euxassay_007488_11	euxassay_007488_12	euxassay_007488_13	euxassay_007488_14

euxassay_007488_15	euxassay_007488_16	euxassay_007488_17	euxassay_007488_18	euxassay_007488_19

euxassay_007488_20	euxassay_007488_21	euxassay_007488_22	euxassay_007488_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7754_wholemount_strong.wlz

7754_wholemount_moderate.wlz

7754_wholemount_weak.wlz

7754_wholemount_possible.wlz

7754_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7754_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9061
Entity Detected:	Cntrob, centrobin, centrosomal BRCA2 interacting protein ( MGI:2443290)
Sequence:	sense strand is shown >T9061 ACAACTTGAAGAAAAAGGATGGGTCTAAGCACATCTTTGAGATGGAAAGTGTTCGGGGTCAGCTCCAGAC CATGCTCCATACCTCCCGTGATACTGCCTATCGAACTGGTTCAGAGAGACGGGAAGAGGACTCCTTTGAC AGTGATAGCACAGCTACCTTGCTCAACACCCGGCCTCTGCAAGACCTGTCTCCATCCAGCTCTGCCCCGG CACTGGAAGAGTTGTTTCCCCGCTACACTAGCCTTCGGCCCGGCCCCCCCACCAACCCCCCAGATTTTCA GGGGCTGAGGGATGCACTGGATGCAGAGCTTACACGCAGAAAGCATTGCGAGCGCCACATCCAGAGCCTA CAGACTCGCGTGCTTGAGTTGCAACAACAGCTAGCTGTGGCTGTGGCTGCCGACCACAAGAAAGATCTCA TGATTGAACAACTAGACAAGACTCTGGCTCGAGTGGTGGAAGGCTGGAATCGCCATGAGGCTGAGCGGAC AGAGGTGCTGCGGGGACTTCAAGAGGAGCGCCAGGCAGCAGAACTCACCAGAAGCAAGCAGCAGGAGACA GTGACCCGCCTGGAACAAAGCCTTTCTGAGGCCATGGAAGCCCTGAGTCGGGAGCAGGAGGGTGCCAGAC TACAGCAGAGGGAGAAAGAGGCCCTGGAAGAAGAAAGGCAAGCCCTGACTCTGAGGTTGGAGGTGGAGCA GCAGCAGTGCCGGACCCTCCAGGAAGAGCGGGATGAGGCTAGGGCTGGGCAGCTCAGTGAGCACCGGAAG CTGGAGGCCCTTCAAGTGGCTCTACAAGAGGAAAGGCAGGCCTGGATCAAGCAGGAACACCAGCTGAAGG AACGCCTTCAGGCACTGCAGGAGGAAGGCCAGGCTCAGCTGGAAAGAGAGAAGGGGAACAGCCAGAGGGA GGCCCAGGCAGCCTGGGAGACCCAGCAGCAGTTTGCACTGCTCCAGACTGAGGTGCGGCGGCTGGAAGGG GATCTGGACACAGTCCGGAGAGAGAGAGATGCACTGCAGCTGGAGATGAGCTTGGTCCAGGCCAGATATG AGAGCCAGCGGATCCAGATGGAGTCAGAGCTGGCTGTACAGCTGGAGCAGCGGGTGACTGAGCGGCTGGC AGAGGCTCAGGAGAACAGCCTGCGGCAGGCCGCCTCCCTCAGGGACCATCACAGGAAGCAGCTACAGGAA CTGAGTGGACAGCACCAACAGGAACTGGCTGCCCAGCTGGCTCAGTTCAAGGTAGAAATGGCTGACCGGG AGGAACGCCAGCAGCAGGTGGCTCAGGACTATGAGCTCAGACTGGCAAGGGAGCAAGCTCGAGTGCGTGA CCTGAAGAGCGGCAACCAACAACTGGAGGAGCAGCGGGCGGAGCTGGTGGAGCGGCTCCAAGCCATGCTG CAGGCCCACTGGGAGGAGGCCAACCAACTGCTCAGTACCACGCTTCTGCCTCCCAATCCGCAGGCTCCTC TTGCTGAGCCCTCCAGCCCCGGGCCTCTGGAGCCAGAGAAGGGGGAGAGGAGGACCTGGGCCATGCCTCC CATGGCTGTGGCTCTGAAACCTGTGTTGCAGCAGAGCCGAGAAGTGAAGGGTGATGTGCCTGGAGCTCCG TCTGTCCTCTGCAGCACCTCCCCAGATCTGAGCCTCCTGCTGGGACCCCCTTTCCAGAACCAGAACTCCT TTCAGCCCTTGGAACCCAAGCCTGATGTGACTCCACCCACAGCTGGGCCCTTCTCTGCATTGGAGGCTTT CACTGATGATCACAGGGCCGAACGCCCGTTTCCTGAGGAAGACCCGGGATCTGATGGCGATGCAAGGCTG CCACCTGCTTCTCAGCTTGAAGGCCTCAAGAACTTTTTGCAACAGCTACTGGAGACAGCGCCTCAGAGCA ATGGAAACCCCTCAGCCGACCTGTTGCTCCCTAAGGCTGGCTCTCGGGCTGTCTCGTCTTGGGAGGAGGC CCCTCAAGTGCCCCGACTTCCACCTCCTGTCCACAAAACCAAAGTTCCCTTAGCCATGGCGTCAAGTCTC TTCAGGGTCCATGGGCTTCCCTCAACCAATTTGCAAGGCAGTGGTCTCAGCACTGGCTCCCCAGAGAAAG ATGGGCTCAACCTGGTGGACGTGTCTGAGCTGTTACGCCTCTACCAGGCTCGGGGTTGGGGTGCCCTGCC TGCTGAGGACCTGCTCCTGTACCTGAAGAGGCTGGAACACAGTGGAACTGATGGCCAAGGGGAGCTTGTC CCCAGAAGAAACACAGACTCCCGTTTGGGTGAGACCACTCGGAAAGAGATCCCCTCCCAGGCTGTCCCTC GACGCCTTGCTTCAGTCCCTAAGACTGAAAAGCCTGCACGGAAGAAAAGTGGGCATCCCGGCCCTAGCAT GAGGAGTCGGGGCGGCATCTGGAGATGAGGCCCGCCCTTCTCCTCTTCTGCCATTCATTTTAATTAGTGC CCTGCATTCTGTCTGTCTCAGAGGAATCTGGAAAATGGAGATTTTTGTAGGAAGCTACTTTGTGAAGAAA CCTTCCAGAATTGTTTTATTATTTTAGAATATATTTGTTTTCTATGAGAAAGCCTATGGAGCCTTTGAGG CTGGTGTGTGACTAGATGTCTGAGTGGCCCTAGGCTCAGGGATGCTTGCAGTGGTTAGATGCCCCATAGT AGAATTATGAGTGAATCTTTTCTTTTTGTATAATTTATATTTTGTATATTTTCTAAAATAAGTATGTATT ACTTTAGTAATAAAAAAAACTCCCAAACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC
Notes:	The probe template was PCR amplified from IMAGE:6817014 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6817014 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7755 same embryo
	EMAGE:7752 same embryo
	EMAGE:7756 same embryo
	EMAGE:7753 same embryo
	EMAGE:7751 same embryo
	EurExpress:euxassay_007488 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source