EMAGE mouse gene expression spatial database

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EMAGE:7755

Tmem163 transmembrane protein 163 ( MGI:1919410)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007485. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007485_01	euxassay_007485_02	euxassay_007485_03	euxassay_007485_04

euxassay_007485_05	euxassay_007485_06	euxassay_007485_07	euxassay_007485_08	euxassay_007485_09

euxassay_007485_10	euxassay_007485_11	euxassay_007485_12	euxassay_007485_13	euxassay_007485_14

euxassay_007485_15	euxassay_007485_16	euxassay_007485_17	euxassay_007485_18	euxassay_007485_19

euxassay_007485_20	euxassay_007485_21	euxassay_007485_22	euxassay_007485_23	euxassay_007485_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7755_wholemount_strong.wlz

7755_wholemount_moderate.wlz

7755_wholemount_weak.wlz

7755_wholemount_possible.wlz

7755_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7755_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9060
Entity Detected:	Tmem163, transmembrane protein 163 ( MGI:1919410)
Sequence:	sense strand is shown >T9060 CGCGACGTGAGTGGGGGGCGCGTCCTCCGTCACGCCCTCACCCAGCCCTGCGCTACCCGCAGGCTGGTCC CCACGCCCCCTAACAGTTCCCTCAGCTTCCACGCGTCTGGACCGTCCGGGGCCCTCCCTCTCCACTCGCC TGGGTGCATCGATACCAGTGCCCGGGCTTAGCGCCTCGGGGCGGGTCCCTGCCGCCCGCACCTGCCCCCG GGGGCGGAGCGCAGCGGCGACAGCGCGGCACCAGCCTCCGCCACTCGGTGTGCTAGCGGCGGACCTGCCG CTCACCGCGGTACGGGCGAAGCTCTCTCTCGGTGCCCTCGGTCTCTGGCACTTTGGACTTCGTGTCTCCA TCGTGGTCGGCTTCGCCTCGGGGGATCTGCAGCCCCGGCGCCATGGAGCCGGCTCTGGGCAGCGAGCGCC GCAGCCCCCCTGGCCCCGGAGTCCCGCGGCCGCCACCGCGGGGCCACGCGCCCTCGACCGCCGCCCCCGC CCCCAGCCCGGCTCCGATGAGCTCCTCTGTGCAGTCTGATGAGGAAAGGCAGCCACGGATCAGCGAGAGC GGCCAGTTCAGCGACGGGTTGGAAGACCGAGGTTTACTAGAAAGCAGCACCCGGCTGAAACCTCATGAAG CCCAGAACTACAGGAAGAAGGCATTGTGGGTATCCTGGCTGTCCATTATTGTCACCCTGGCCCTGGCAGT GGCCGCCTTCACTGTCTCCGTCATGAGGTACAGCGCCTCTGCCTTCGGGTTTGCATTTGATGCCATCCTG GATGTGCTGTCCTCGGCCATTGTCCTCTGGCGTTACAGCAATGCGGCTGCTGTGCACTCTGCAAACAGGG AGTATATAGCCTGTGTCATCTTGGGGGTGATCTTTCTTCTGTCATCTATATGCATAGTGGTCAAAGCCAT CCATGACCTCTCAACTAGGCTGCTCCCTGAAGTGGATGATTTCCTGTTCAGCGTCTCCATTCTAAGCGGG ATCCTTTGCAGCGTACTGGCTGTGCTGAAGTTTATGCTGGGGAAGGTCCTGACAAGCAGGGCTCTCATCA CAGACGGGTTCAACTCCCTTGTTGGGGGCGTGATGGGCTTCTCCATCCTTCTAAGTGCAGAAGTGTTCAA ACACAACGCAGCAGTCTGGTACCTGGACGGCAGCATTGGTGTCCTGATTGGTCTCACCATCTTTGCCTAC GGGGTCAAACTCCTCATCGACATGGTGCCCAGAGTGAGACAGACGCGTCATTATGAAATGTTTGAGTGAA GGCAGCAGGAAGGGCGGCAGGGACTGCCAGCCCCGATGAAGAGCGTCACTTGACAAGAGGTTTCCACGCA GGCAGATGGTGCCAACATTCAACTGCACATCCAGTTTCATTTTGGGAAGTTTTCGTTCATAGAGTCTGTC ATCATGGGCTGGGGTCTGCCAGAGGCTGGACCTGCCCTACATCCTCACCCAGTCGCCCACCATCAAACAT GTTAGGATGACGTTGCCCGTGGGAGGGGCCGCCATGCTTCCCAACCTTGAGCCGGAAGTGACATTTTTCT ATTTCCTGGAGTCAAACAGTTCTTGGAGGCAGGCTAGGGTCTCTGCTTGACAGGAAGCCTGTGGTGCACA CTGTCAGCCAAAACCAGATAGTAACTGACTGGGTCACCTCCACCTGACATGTGGCCGAACCACCTCTAGA GTCACACTCTCCTAGCAGATGCCATCGGTACAGCACTTCCTAACTCCTACCAACACGTTTCCTACACTTT TACTAATCCCAACACTAAACCGGTTCGTTCAGAAGGAGGCCATTCTATTTTTTAAAGTGTTTAGTGATCT TGAGCTTTGCATGGACAAGCTACGTAAGCACACAGCCCTTTCCTTCACGTCTAGAACCCACACATCTGTA AAAACCCAGGAGCCATTTTTGAGAGATGTGAACCTCTAAACATTTATTTGTAATAAATAATTATAATCAT CACCCCGAATCTGCATATCTCTATATGCTGGTCACAGTACCTCAGTCTGTGAGACGTGGGCCGCTCCTTC AGAAGGAACAAGATGCCCTCAGTTTATGTAACATTAGATATGTGCTGTAGACATGCCCTCATTCCACCAT GTTCTGTAGCTCTCACAGTACTGCCCACACGGGCAGCCATGTCAGGCCTATAAAGACCTTTACCATTTGT ACGATGTGGGGAGGTTGTATTTGAGAGCTGGTAGTGTGTGTGCCAACATGTAGGGAGTACACGTTGTGGG CAGGCTCGCTCTCTCTCTCTCTCCTCTGTTTCCTTTGTGTGTTCGCATGTGGAGTTTGTTTGTTTATTTA TTTATGAATGAAACAGTCCCTCACCCAAACTAGAGCTCGCTGGTTCAGCGAGCCTGTGAGTCTTGGGAAT CTGTTTATCTTGTTTTCCCAGTACTGGGGTTTCACCATCAGAGGCGGCTTCCTGTGGGTGCTGTGGAGCT GACATCAGGTCTTCCTTTTTGTACAGCAAGTGTTCTGCTCAATGAGTCATTCCCCCATCTCCAGCAGGCA ACTCGAATGAGAGGAGCCCATTTCCAGCTCCAGGCTGCTGGCTTCCTGAAGGACTACAGACCTATTCCTG CCATCTGTCTACTCTAACGGACACACAGACAGAGGGGAAGAGAAGAAAGAGAAATTAAAAGAGTTTTAAA TAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816957 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816957 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7754 same embryo
	EMAGE:7752 same embryo
	EMAGE:7756 same embryo
	EMAGE:7753 same embryo
	EMAGE:7751 same embryo
	EurExpress:euxassay_007485 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source