EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7759

Elfn1 leucine rich repeat and fibronectin type III, extracellular 1 ( MGI:2442479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007454. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007454_01	euxassay_007454_02	euxassay_007454_03	euxassay_007454_04

euxassay_007454_05	euxassay_007454_06	euxassay_007454_07	euxassay_007454_08	euxassay_007454_09

euxassay_007454_10	euxassay_007454_11	euxassay_007454_12	euxassay_007454_13	euxassay_007454_14

euxassay_007454_15	euxassay_007454_16	euxassay_007454_17	euxassay_007454_18	euxassay_007454_19

euxassay_007454_20	euxassay_007454_21	euxassay_007454_22	euxassay_007454_23	euxassay_007454_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7759_wholemount_strong.wlz

7759_wholemount_moderate.wlz

7759_wholemount_weak.wlz

7759_wholemount_possible.wlz

7759_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7759_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

strong

regional

strong expression: see section 11 12 13 16 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23 24

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 08 09 10 13 14 15 16

facial vii ganglion

moderate

single cell

moderate expression: see section 05 06 22

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 08 09 19 20

trigeminal v ganglion

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

dorsal root ganglion

moderate

regional

moderate expression: see section 18 19 20 21 weak expression: see section 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8985
Entity Detected:	Elfn1, leucine rich repeat and fibronectin type III, extracellular 1 ( MGI:2442479)
Sequence:	sense strand is shown >T8985 AGGCAGTGATCCATCTCACCCCTAAGTCCCCTGGGGGGGCCCACTGCCCAGCCCTCCCCCCATCCCCACA GGCAGTGTCCCGAGATGGCTGGGCACGGGTGGGGTACAGCGTGGGTACTTGTGGCAGCCGCCACGTTACT GCACGCGGGTGGGCTGGCCCAAGGTGACTGCTGGCTGATTGAGGGTGACAAAGGCTTCGTGTGGTTGGCC ATCTGCAGCCAGAACCAGCCACCCTATGAGGCCATCCCCCAACAGATCAACAACACCATCGTGGACCTGC GGCTTAACGAGAACCGTATCCGCAGCGTGCAGTACGCCTCCCTGAGCCGCTTCGGAAACCTCACATACCT CAACCTCACCAAGAACGAGATCGGCTACATCGAGGACGGGGCCTTCTCCGGCCAGTTCAACCTCCAGGTG CTGCAGCTGGGCTACAACCGGCTGCGCAACCTCACGGAGGGCATGCTGCGGGGCCTGAGCAAGCTCGAGT ACCTGTACCTACAGGCCAACCTCATCGAGGTGGTGATGGCCAGCGCCTTCTGGGAGTGTCCCAACATCGT CAACATAGATCTCTCCATGAATCGCATCCAGCAGCTGGGCAGCGGCACCTTCGCAGGCCTGACCAAGCTC TCGGTGTGTGAGATCTACAGCAACCCTTTCTACTGCTCCTGTGAGCTGCTGGGTTTCCTGCGCTGGCTGG CTGCCTTTACCAACGCCACACAGACCCATGACCGGGTGCAGTGTGAGTCTCCTCCTGTCTACGCAGGCTA CTTCCTCCTGGGCCAGGGCCGCCACGGCCACCAACGTAGCATCCTTAGCAAGCTTCAGTCTGTGTGCACA GAGGGCTCCTACACTGCTGAGGTGCTTGGCCCACCCCGTCCGGTGCCAGGCCGGTCACAGCCGGGACATT CGCCACCTCCGCCTCCACCAGAGCCTAGTGACATGCCCTGCGCTGATGACGAGTGCTTCTCCGGAGATGG CACCACGCCGCTGGTGATCCTGACTACGCTGGTCCCTCAGACAGAGGCCCGTCCCTCCATGAAGGTCAAG CAGCTGACCCAAAATTCGGCCACCATCATGGTGCAGCTGCCGAGCCCGTTCAACCGCATGTACACGCTGG AGCAGTACAATAACAGCAAATCGTTCACCGTGTCCAAGCTGACCCAGCCTCAGGAGGAGATCCGCCTCAC CAATCTTTACACACTCACCAACTACACATACTGCGTGGTCTCTACCAGCTCCGGCACCCATCATAACCAC ACCTGCCTTACCATCTGCCTGCCCAAGCCGCCCAGCCCACCGGGCCCCGTGCCCAGCCCCTCCACAGCCA CCCACTACATCATGACCATCCTGGGCTGCCTCTTTGGTATGGTACTGGTTCTGGGCGCTGTCTATTACTG CCTGCGCAAGCGGAGGCGACAAGAGGAGAAACACAAGAAGGCTGTGGCAGCGGCAGCTGGCAGCTTGAAA AAAACCATCATCGAGCTTAAGTATGGTCCTGAGATTGAGGCGCCTGGCCTGGCTCCCCTGACCCAGGGTC CGCTGCTTGGCCCTGAGGCTGTGACTCGAATACCCTACCTGCCAGCCGCCACCAGTGACGTGGAGCAGTA TAAGCTGGTGGAGAGCAGCGAGACCCCCAAGGCCACCAAGGGGAACTACATTGAAGTGCGCACGGGGGAG CCGCAGGAGCGCAGAGGCTGTGAGCTGAGCCGGCCAGGTGAACCCCAGAGCTCAGTAGCGGAGATCTCCA CCATTGCCAAGGAAGTGGACAGAGTCAACCAGATCATTAACAACTGCATCGACGCCCTCAAGTCCGAGTC CACCTCCTTCCAGGGCGCCAAATCAGGGGCCGTGTCTGCGGCTGAGCCGCAGCTGGTGCTGCTGTCCGAG CCTCTGGCCAGCAAGCACAGCTTCCTGTCCCCTGTATACAAAGATGCCTTTGGCCATGGTGGCCTGCAGC GGCACCACAGTGTGGAGGCTGCGCCCGGGCCCCCGAGGGCCAGCACTTCATCCAGCGGTTCTGCACGCAG CCCTCGCACCTTCCGGGCTGAGGCCACCGGCACACATAAGGCCCCAGCCACCGAGACCAAGTACATTGAA AAAAGCTCACCTGTGCCCGAGACCATCCTCACTGTGACACCCGCGGCCACTGTGCTGCGGGCGGAGGCCG ACAAGAGTCGCCAGTACGGCGAGCATCGGCACTCATACCCCGGCTCTCACCCTGCCGAGCCACCTGCCCC TCCACCACCTCCACCCACTCACGAGGGCTTGGGTGGCCGCAAGGCATCCATCCTGGAGCCTCTGACCCGG CCACGACCCCGAGACCTCGTCTACTCGCAGCTGTCCCCACAGTACCACAACCTGAGCTATTCATCCAGCC CCGAGTACACCTGCAGGGCATCCCCAAGCATCTGGGAGCGTCTCAGACTGAGCCGACGGAGACACAAGGA TGATGCGGAGTTCATGGCGGCTGGCCACGCCCTGCGCAAGAAGGTTCAGTTTGCCAAAGACGAGGACCTG CATGACATCCTAGACTACTGGAAAGGCGTGTCTGCCCAGCACAAGTCCTGAACCTCCCTCCACCCTTTGG CAGGGCCCAAGGAAGCCTGGCCCTGTCCTCTCCGAAACTCGTGATGCCCATTGGACCAAAAAGGATAGAC CACCCACAGCAGCTATCCCTGACTGAGACTTAACACTAAATCCAACATATGTGCACAACCAGGGGCCGCT GCATACAGCACCTGGCCCACAGGGACAGCTTGGGGAGGCCGCAGGTGGCCGAGACAGTAGATATACTAAC TTCCATGTAAACTGTAATGTGCCTGCTGCCTGGTGCAAGCAAGCAAGACACACACACACACACACACACA CACACACACACACTCACACACTCACATACTCACATACACTCACACATTCACACACTTACACTCACACATA CATTCACACACACAAATGCACACACATGCATGTGCGCGTGCACACACAAACATTTGCACACACTCACACA TGCACATGCACACACATGCACACATGGGACTTCGGAAAACTGTGTCTTACGGGTGGGGGTGGGGGAGGGG AAGTTTTTCCATTATCTTTCCTGTTTTGAGTCTTCTCTTCCTTTTCCTTCTTCTCTCTCTTTATTTCTTC CTTTTTAAAAAATATATATATAAAAGTGAAAATCTTTAAAAAAAATAAAAGAGAGAGAAAGAAGAAAGAA CTAAATCACCTAGCGTGGGGCATGTGACCAAGTCTAGCCAGCTTCATGGCACCCCCTAAATATGATCTCC CAGGCTGTGGAGTGCAGAGGGGTGGAGCATCTGGAGCCTGTGAGACTCTAGTGGGGAGGTGAAGACTGTG CCTGGGGACCAGTCAGGGTGGCAAGAGGGGTGCAGGCTGGGAAACATTGTGTGGTCCCTCCACCTCGACC CCATCTTTATTGTACACTGTAGCTGTTCTATTGTACAGAAAAAAAAATCTATATTTGCAAAGTTTGCTGT AAAATGAGAGAGAGAAAAAAAGACTATGTCTACTAAAAAAAATCTGCAAAGGAAAAAAAATCCAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811341 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811341 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7758 same embryo
	EMAGE:7760 same embryo
	EMAGE:7761 same embryo
	EMAGE:7762 same embryo
	EMAGE:7757 same embryo
	EurExpress:euxassay_007454 same experiment
	MGI:4824522 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source