EMAGE mouse gene expression spatial database

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EMAGE:7761

Pppde2 PPPDE peptidase domain containing 2 ( MGI:106313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007463. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007463_01	euxassay_007463_02	euxassay_007463_03	euxassay_007463_04

euxassay_007463_05	euxassay_007463_06	euxassay_007463_07	euxassay_007463_08	euxassay_007463_09

euxassay_007463_10	euxassay_007463_11	euxassay_007463_12	euxassay_007463_13	euxassay_007463_14

euxassay_007463_15	euxassay_007463_16	euxassay_007463_17	euxassay_007463_18	euxassay_007463_19

euxassay_007463_20	euxassay_007463_21	euxassay_007463_22	euxassay_007463_23	euxassay_007463_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7761_wholemount_strong.wlz

7761_wholemount_moderate.wlz

7761_wholemount_weak.wlz

7761_wholemount_possible.wlz

7761_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7761_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8988
Entity Detected:	Pppde2, PPPDE peptidase domain containing 2 ( MGI:106313)
Sequence:	sense strand is shown >T8988 GTTGCCGGTCGCGGGTGTCCGGGCGCGAGCAGCGCAGGGCCGCCTCTCCCCCGCCCTCAGGCCGGGCCCC GGCTCGGTCCCCCGCTCCCTCTTCTCCCCACCCCGGTCGGGTACCGGAAGGTCCAAGCCGCTGCCGGGTG CCCGAGGGCCATCGTGGCCGCCGCCACGGGTCCTGATGGAGCCGCCAAATCTCTATCCGGTGAAGCTCTA CGTGTACGACCTGTCCAAGGGCCTGGCCCGCCGGCTCAGCCCCATCATGCTGGGGAAACAACTGGAAGGC ATCTGGCACACCTCTATAGTTGTACACAAGGATGAGTTCTTCTTCGGCAGCAGCGGTATTTCCAGCTGTA CCCCGGGAGGGACATTGCTTGGGCCCCCAGACTCTGTGGTTGATGTGGGAAACACAGAAGTCACAGAAGA AATCTTTCTGGAATACCTGTCCTCCCTGGGGGAGTCTCTGTTCCGAGGAGAAGCCTACAACCTCTTTGAA CACAACTGTAACACCTTTAGCAATGAAGTGGCGCAGTTCCTGACTGGGCGAAAGATCCCTTCTTACATCA CCGACCTGCCCTCAGAAGTTCTTTCCACGCCCTTTGGACAAGCACTTCGGCCCTTCCTGGACTCCATTCA GATCCAGCCTCCAGGAGGGAACTCTGTGGGCAGACCCAATGGCCAAAGCTAACAGGACTGCACGGGACCA CCCACCTCGCCAGGGCTTTTCCTTTTTAAACAAAACAAACCCTACCAGATTTCTATTTTATAATTTTACA TCAGAGCTAACAGGGACGGCTTTTTAAATTTCCCAGGGAAGGAGTTCTTCAGGCTGCATGTAGGACAATG CTCCTGAGAAAGATCGAATGCCATCCCTTCTAATAGTATTATACTAATTTATTAAGGCTGTCTTGTCTGT GAGTTCACTTTTGACGCTGTTGCCTAAGCGTCCTCCACACTGGAGAGAGAGGGTGCTTGTTTAAAGAGAA AGCAAGAGTCACAGTCTGGGAGAACCCCATCTCGGACCCTCTTTCCAGAGAACACCCACATTCCACCCAG GTTGCCACCCTGTGGGACCCAGGTGAGTTTTATACTGTCCATTCCCCTGGAGCAGTCACCTTCTAGCAGT AACTGAAGCAAAGGACTTGGAGTACATGGGGCAGCTGGAGGCTCCTCACTCAAGCTAAGTGACCTCTTTA ACCACAAGTTTACCCTCCTCCCGAGACAAGGCCACTCATCACAGCCCAGCCGGAGCAGCCGCAGCGTCAG GATCAAAGGAGACTGGCAGCAGGTGTGAGGCCAGAGAAAACAGGAGAAAGCCATTCCTCCTCCTCCTCCT ATCTGAGTGCCAACCCTCGATGCCCACCCCTAAGCTGCTCTTCCTCAGCGTAGCTTGACCTGTCACCTTT AATAAAGGTCTAGGCTGCAGCAGCAGAGACACAGGCCTTCTGTCTGAGAATTAGTTATTTATAGTCAATA GAGCCAGCCACTCACACCCTTATGGGTTTGTCAGCTAGGGACTAGGGGGACATGATTGTTCTCCCAAAAC AAGTCTCTGATGTAGAAAATACCATAATGATGTAGATTCATGGTGGGAAGAAAATTTGGAGTTGTCTCCA GTAGGGACTTTCACATTATCCTTAATACGTCTCCATTGTCATTCTGACCTTATGGGGTGTGGTTGGGGTG TGAGTGGGCGTTTTCTTAATGATATGCTGCTAGGATTTCATGGGCAGCAATAGCCTAGAAGTCTATGATC TAGACTCCAGATTCCAGAGGGACCTTAAGGTTCCCTTCTGTGGTGTCAGTCTTCTACTAGCTAATATGGG AAACTGGATATGGAGAAGGGAGCTCAGAGCAGGCACCTGAGACTCAGCTCCACACCCATGGGGAGCCCCC CACCGTGGCTTCCTGCCACTTCTGAAACTTGAGCCCACTTGTTCCTTTTGGGGTAGGCCTTTCCTCCCTG AAAAGCCCCTATTGGCTCCCTTGCCCCTACCCTGCAGGCTTTGCCACTAGCCATAGCAGTGGCATCATGG AGATCTCTGCCTCAGAGCTGACCTGTTGTCTGACCAGAGAGAGCTATGTCCACCTAGGGTGTCGAGTGGT GTTTGGGAACTTGAAGGTCACAGTCAGAAAGGTCTCAGGACTCAACAGTGTACAGGTGTATGTGGCTGTC ACAACGTAGAGTGGAAACCAACAGAGACTGTTTAGTGTTGGTCTCTTTGGCAGAATAGGGCTTAGAAGGT TCAGTGTTTAAAGCTTGGTATCTGGGAAGGCAGAGGTTGCTGGAAGCCATCACTTTTTCCTTTGCCTGGC CCCAGCTGGTAGCATCTCTGCCCAGAGCTCTGTCCTTAGGGTGCTCTCACATGGCCATTCTCTGTTTTTC TCCTCAGCAGTAACCATGGTATTCTGAGACATTTGCCTGAGGATTTTAGTCACTTAATTGCTTGAATTAT CTCCTGTCATGTCTAGCAGCCATCCATGCCAACGTCTGTGGCCATGGCTAGCAGAGAGATGGGAAGACTG GACAGTCTCTCTGGTCTCCTTTCCCTATGCAAGTTCCGTTGTCACCAGAATCCCAGGCCAGGAACCCCAC ATCCCACCTTTACATTTCTCTGTCCCAGTACCTCAGGAGCATACTGTTCATGAGCAGGTGACTCCCCCTG GGCATGTACAAGCAAGCTGAGAGGAAAAGGAGTCACGTGGTTCATGCCACATAGTGGACAGCCCTGTCCA TTGGTCAGTTGGTCAAGGGACAAGGGAGGTGGAGGCATGAGACCCTTGTCCGCTCCTAGACTGAAGGGTT TCTTTGAGATTCCTTCATGACTTCCTTGAAGTCAAACTAATGTCTAATACGGAGGTGTTTGCTGCTTTTC ATTGGTGTTTTTTCTAATGCAATAAACTCATGTCTGCCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811343 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811343 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7758 same embryo
	EMAGE:7760 same embryo
	EMAGE:7759 same embryo
	EMAGE:7762 same embryo
	EMAGE:7757 same embryo
	EurExpress:euxassay_007463 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source