EMAGE mouse gene expression spatial database

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EMAGE:7762

Diras1 DIRAS family, GTP-binding RAS-like 1 ( MGI:2183442)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007464. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007464_01	euxassay_007464_02	euxassay_007464_03	euxassay_007464_04

euxassay_007464_05	euxassay_007464_06	euxassay_007464_07	euxassay_007464_08	euxassay_007464_09

euxassay_007464_10	euxassay_007464_11	euxassay_007464_12	euxassay_007464_13	euxassay_007464_14

euxassay_007464_15	euxassay_007464_16	euxassay_007464_17	euxassay_007464_18	euxassay_007464_19

euxassay_007464_20	euxassay_007464_21	euxassay_007464_22	euxassay_007464_23	euxassay_007464_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7762_wholemount_strong.wlz

7762_wholemount_moderate.wlz

7762_wholemount_weak.wlz

7762_wholemount_possible.wlz

7762_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7762_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16

hindbrain

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

strong

regional

strong expression: see section 05 06 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 19 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 20 21 weak expression: see section 19

spinal cord

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 16 17 18

cervical ganglion

weak

regional

weak expression: see section 10 18

thoracic ganglion

weak

regional

weak expression: see section 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 18 19 20 21

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8984
Entity Detected:	Diras1, DIRAS family, GTP-binding RAS-like 1 ( MGI:2183442)
Sequence:	sense strand is shown >T8984 GCGGACAGCAGCGCAGCGGCGGGGACCACAGCGGCCCAGGGTCGGTCTGGGGTTCTAGCTGCCGGCCCTG TCCTCCAAGCCGGCTCCGGAGAAGATGCCAGAACAGAGCAATGACTACCGTGTGGTCGTGTTCGGCGCAG GCGGCGTGGGCAAGAGCTCGCTGGTGCTCCGCTTTGTAAAGGGGACGTTTCGTGACACCTACATCCCTAC CATAGAGGACACGTACCGGCAGGTGATCAGCTGTGACAAGAGCGTGTGCACACTGCAGATCACGGACACC ACCGGCAGCCACCAGTTCCCGGCCATGCAGCGGCTGTCCATCTCCAAGGGCCACGCCTTTATCCTGGTGT TCTCGGTGACCAGCAAGCAGTCGCTGGATGAGCTGAGCCCCATCTACAAGTTGATCGTGCAGATCAAGGG CAGCGTGGAGGACATCCCCATCATGCTGGTGGGGAACAAGTGTGATGAGACGCAGCGGGAGGTGCACACG CGCGAGGCGCAGGCCGTGGCACAGGAGTGGAAGTGCGCCTTCATGGAAACCTCAGCGAAGATGAACTACA ACGTGAAGGAGCTGTTCCAGGAGCTGCTGACGCTCGAGACGCGCCGGAGCGTCAGCCTCAGCGTGGACGG CAAACGCTCCAGCAAGCAGAAGAGGGCTGACCGCATCAAGGGCAAGTGCGCGCTCATGTGAGCCCGCCAG AGCCAGCTTGAGTCTCCCGGGCTCCTTCTCCCCTCCCTCCTCCCTTCCCTCCTCTCTCCTCTTAGTATTC CTCTCCTCCCCACCTCCTCCTCTCTCCTCCTCTCTGCTCCTCCCTTCTTTGCTCCTGCTCCTTCTCCCCC TCCTTCCTTCCTTTGCCTTGCCCCTCCCTCCTCCATCTCTCTTCCCTCTTCCTGTCCCCACCAGCCTACA GTAAGGTTGGGCTCCATCCCTGCCTCTTCACACCTCCATAGCTTCATCCTTTGCAGACCTCTCCCTCTAT ATCTCTTTGCTCCCCCACCCCCATTCTATCTGCACCCCACAACCACACACTGGAGGTTTGATCCCTGTGG ATCTGAAAACAGGGACCTGGCTAAGGAACTGCTCATCCCAGTCAGGCCCAGAGAGGTTACCTTGCCTCTC CATCTCTGTCCCTGTGTGCCCAGCCATGCCCACTGTCCCTGTCCCTCACATTCTATCACTGTGACCTCTG GGGAGTGCCCATTGACCTCATCTTCCTTCACTAATATCTTATTTCTTTCACCTGATCCTGTCCTTTCCCC GTGACCTCTGACCTGTGGCCTTCCCTGGGTCCAGAACACCCCTGTCCTCTCCTGTCTGCACACCAGGGTG GATGGGCCCGAGGGCTGGGCCTCAGGCACCAGGCAATAAACACAGGGCCTGCAGCAATGCCCTGGCCACC CCGAGTACCACCTCTGGAATAGCAGACGCCCACAGCACAAGCCCAGGACTGTCCCTTCAGGGGAGGCACA CTGGAACCCTGAACTTGGAGTGTGGAGTGGCTGCCTGGACACTGCCCACCTGGACACCAGCCTTCTGCTG AGATAGAGACCTTGGCACAGTGGCACCTCGACAGCCACCCCGACTGGGGGTTTGGGGTGCTGAGGGTGGG GTGCCCAGCTGAGAGTCTATCAGCAAAGTCCCTGTGTGCGGTGATAGGACTAGTGGCTGCTGCCCTAGGG CTCTGACAGAATCTGAGCCTCTTTGCTGGTCATGTGTACTCTGGAGACTGTCCCCCATGTCTGCCCTGAG TTACGGAGCCCCTGTGGTATCTGCGAGACTGTCCAGGAGACTTGAGTGGCTCCTGGGCCTGTGTCCCACC CAGATGAGGGTGACTTAGAATTCTGTGTTTATGTGCCTGTGTGTGTGGATGGGTGTGTGTCTGTCTTGGG ATGGGGTGTTCTTGCACCACTTTGTGTTCTTAGTGAGACCACAGTGTAAGTGGCCGTGTGTGTGTGTGGG GGGGGGCACCTAGACATTGTGGTGTGTCTGTATGGCTCTGTGTTTTCAGGGGTGGGTGACACAGGCCTGT CTGATGAGCACCAGGTCTGTTCCAGGCTGTGGCAAGCTGGCTCCTGGCTCAGAAGTGACAAAGCTGCAGT ACAGAGGCCTCAGTGTGAGGTCATCACCTCACAGTGGCTACAGACCAGGCTGTGGCCTGAGTGAGCTGGA ACCTCTGGGGTGGGGACGAGAAAGAGAGAGGGAGAGAGAGGGGGGAGAGTGAGTGTGTGTGTGTGAAGGG GGAGAGTGTGTGGGGGAAGAGTGAGAGAGATAGAGTGTATGTGTGAGAGGGAGGGAGAGTGGGAGGTAGA ATGAGAGAGAGTGTGTATTGGAAGGGAGAGAGAGAGTGTGAGAGAGTGTGAGAGAGAGACAGAGACAGAG ATAAAGGAGATAAAGAGAGACAAAGACATGGTCACAAAGAGACAGAGAAGCAGAAAGAAGGCTGAAGCCA GATGACCAAGTAGGCCGGACCCCACTGTGTTCCCTTCTGTGTGCCAGGCACAGGGAGAGCCACAGCCTGA AGCCTGAGTCTGAGCATAGCTGAGCCCTGACCTCTAGCCAGCCCAGGGCCCCCACAGCTGCCACCTGCTC CCAAGCCCCGGGAGTGGCTCCTGGCTGGACAGGGGGTCACAGCCCTTTCTCAGGGACACACCCTGATAGC ACAATCTCTGGGCCACCCCAGGCTTCTCCTGGCTGGCCTTGCCCATCCTGGGGAGAACCGGGCTGGGTGG CAGGGGAGGCCGGAGCCCACCCTGGCTGCTGGCCAACCTGCCCAGGCATCTCTGGTGCTGGGGACCCCGC CAGGCCTCGCTTGCTGTGACCCACCCCTGCCCCCCGCATGTGGGAGCCCCATCCCATGTGGGGTCTGTGT ACCGTTCTCTCGTAGACCTTGTCTTCCTGCAATAAACACGTGGATCCTGCAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810812 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810812 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7758 same embryo
	EMAGE:7760 same embryo
	EMAGE:7759 same embryo
	EMAGE:7761 same embryo
	EMAGE:7757 same embryo
	EurExpress:euxassay_007464 same experiment
	MGI:4824310 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source