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EMAGE:777

Hoxb1 homeobox B1 ( MGI:96182)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:777 EMAGE:777 EMAGE:777 EMAGE:777
Figure 5C of Frohman et al., 1990 [PMID:1983472] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 5 of Frohman et al., 1990 [PMID:1983472] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 5A of Frohman et al., 1990 [PMID:1983472] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 5B of Frohman et al., 1990 [PMID:1983472] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Abbreviations used: al, allantois; am, amnion; ar, archenteron; ec, ectoderm; en, endoderm; me, mesoderm; ps, primitive streak. Only the data from Figs 5B/C has been spatially mapped in this EMAGE entry. The section plane is indicated in the cartoon.
Expression Pattern Description
Spatial Annotation:
EMAGE:777EMAGE:777Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
777_voxel_strong_3D_1.wlz
777_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:777_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive streak
detected detected
Transcripts are observed along the length of the primitive streak.
embryo mesoderm
detected detected
regionalHoxb1 transcripts are detected in the mesoderm in the posterior half of the embryo.
embryo ectoderm
not detected not detected
Hoxb1 transcripts are not detected in embryonic ectoderm outside the primitive streak.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1331399
Entity Detected:Hoxb1, homeobox B1 ( MGI:96182)
Sequence:sense strand is shown

>MGI:1331399
AGGCCCCCCAGGTTGCCCAAAGGAAGCCGCTGGAGATGCCTCTGACCAGTCCGCGTGCACCTCCCCAGAA
GCCTCGCCCAGTTCCATCACCTCTTGAATTGAACTTCCTAAGTAACTGGGCTTCCAACGCTTGACCAGTT
CTCTCGAAGACTTTCCCAAACTTCACAGCCTTGGTGACCCTCCTCAAGGCCGAGGCACCAGTTTAGAGCT
GCCCAGGAAACTGGGCAGGAGTTGGGAAATGTATTTTTCTCACTCTCAGATCTAGGGGTGGAGGGATGAT
TGATGGCTGGGGATCCTACAGGTCTTGGGACCTGGGGAACACTCAACTCATCAGAGGTCGAAGGAAGGCC
TTTTGGCTTTGATCTGGAGTCAGCCCATCCTTTCGGGCTTCTCCTTTCCCTTCCAACTCAGTTCAGTGCC
TTTGAGCTTAGAGAGTTCTTCTTTCGAATTCTTAGCCCAACTCTTCTTTCTAGGTCTGGGATGTCTAGGC
TGGGAGTCCTTGGGGTTTTTCTGGGGGAGGATGCGTTTGAGTGTGAGCCCAATCGCCACCAACCCTTAAT
CTTAATCCAGCCAACTCACCGCAGACCTTACAATAGCAGACCTTACAATAGCACCGACTGCCTTTGACAT
ACC
nt 868 - nt 1500 of NM_008266.3
Notes:The Hoxb1 (Hox-2.9) probe used in this study by Frohman et al., 1990 [PMID:1983472] is a "633 bp Pst1-SalI fragment" (see Figure 3 therein). The SalI restriction site is located in the polylinker at the 3' end.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Frohman et al., 1990 [PMID:1983472] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1983472] Frohman MA, Boyle M, Martin GR 1990 Isolation of the mouse Hox-2.9 gene; analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm. Development (110):589-607
Links:MGI:1340628 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI