EMAGE mouse gene expression spatial database

EMAGE:78

Shh sonic hedgehog ( MGI:98297)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2.G, Epstein et al., 1999 [PMID:9847242] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

detected

regional

Expression is restricted to the zona limitans intrathalamica (ZLI)

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:19210
Entity Detected:	Shh, sonic hedgehog ( MGI:98297)
Notes:	Probe used in this study by Epstein et al., 1999 [PMID:9847242] is indicated as being described by Echelard et al., 1993 [PMID:7916661] ie. "either a 2.8kb or 0.6kb RNA transcript generated by T7 (2.8kb) or T3 (0.6kb) transcription of XbaI and HindIII digests of Bluescript subclones of the original Shh cDNA inserts". Unable to ascertain which was used here.
Chemistry:	RNA
Strand:	antisense

Specimen

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Epstein et al., 1999 [PMID:9847242] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9847242] Epstein DJ, McMahon AP, Joyner AL 1999 Regionalization of Sonic hedgehog transcription along the anteroposterior axis of the mouse central nervous system is regulated by Hnf3-dependent and -independent mechanisms. Development (126):281-92
	[ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:	MGI:1327566 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source