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EMAGE:7816

1700029F09Rik RIKEN cDNA 1700029F09 gene ( MGI:1922873)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816
"Pseudo-wholemount" of euxassay_007574. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007574_01 euxassay_007574_02 euxassay_007574_03 euxassay_007574_04
EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816
euxassay_007574_05 euxassay_007574_06 euxassay_007574_07 euxassay_007574_08 euxassay_007574_09
EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816
euxassay_007574_10 euxassay_007574_11 euxassay_007574_12 euxassay_007574_13 euxassay_007574_14
EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816
euxassay_007574_15 euxassay_007574_16 euxassay_007574_17 euxassay_007574_18 euxassay_007574_19
EMAGE:7816 EMAGE:7816 EMAGE:7816 EMAGE:7816
euxassay_007574_20 euxassay_007574_21 euxassay_007574_22 euxassay_007574_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7816Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7816_wholemount_strong.wlz
7816_wholemount_moderate.wlz
7816_wholemount_weak.wlz
7816_wholemount_possible.wlz
7816_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7816_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35373
Entity Detected:1700029F09Rik, RIKEN cDNA 1700029F09 gene ( MGI:1922873)
Sequence:sense strand is shown

>T35373
ATCTGCTTTTCAGAGAGATGCCCTTTGCTTCTCAGATGTAGTGCACCTTACGCTTATTTTTCAACAGTGA
AAATGAGTCATACAGAGGTTAAATTAAAAATACCTTTTGGAAATAAATTACTGGATGCCGTTTGTTTGGT
ACCTAACAAGAACATAGCATATGGAATAATTCTTACACATGGAGCATCAGGAGACATGAATCTTCCTCAT
CTGATGTCACTGGCATCCCACCTTGCATCTCATGGGTTTTTTTGCCTGAGATTTACTTGCAAAGGCCTTA
ATATTGTACATAGAATTAAGGCATATAAAGCAGTTTTGAATTACCTGAAGACCTCAGGAGAATACAAACT
TGCTGGTGTTTTTCTTGGAGGTCGTTCAATGGGCTCAAGGGCAGCTGCTTCTGTGATGTGTCATACTGAG
CCAGATGATGCTGATGATTTTGTTCGAGGTCTCATTTGTATTTCTTACCCACTGCACCATCCAAAGCAGC
AGCATAAACTCAGAGACGAAGATCTCTTTCGTATAAAGGATCCTGTACTGTTTGTGTCGGGCTCAGCAGA
TGAAATGTGTGAAAAGAACTTGTTGGAGAAAGTAGCACAGAAAATGCAAGCCCCTAGTAAAATTCATTGG
ATTGAAAAGGCAAATCATTCCATGGCAGTGAAAGGACGGTCTACAAATGATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75572. Forward Primer - name:075572_F_cDNA_1700029F09Rik, sequence:ATCTGCTTTTCAGAGAGATGCC; Reverse Primer - name:075572_N_SP6_cDNA_1700029F09Rik, sequence:CATCATTTGTAGACCGTCCTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7815 same embryo
 EMAGE:7817 same embryo
 EMAGE:7819 same embryo
 EMAGE:7818 same embryo
 EMAGE:7820 same embryo
 EurExpress:euxassay_007574 same experiment
 MGI:4822628 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS