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EMAGE:7841

Ash1l ash1 (absent, small, or homeotic)-like (Drosophila) ( MGI:2183158)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841
"Pseudo-wholemount" of euxassay_007724. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007724_01 euxassay_007724_02 euxassay_007724_03 euxassay_007724_04
EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841
euxassay_007724_05 euxassay_007724_06 euxassay_007724_07 euxassay_007724_08 euxassay_007724_09
EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841
euxassay_007724_10 euxassay_007724_11 euxassay_007724_12 euxassay_007724_13 euxassay_007724_14
EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841 EMAGE:7841
euxassay_007724_15 euxassay_007724_16 euxassay_007724_17 euxassay_007724_18 euxassay_007724_19
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euxassay_007724_20 euxassay_007724_21 euxassay_007724_22 euxassay_007724_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7841Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7841_wholemount_strong.wlz
7841_wholemount_moderate.wlz
7841_wholemount_weak.wlz
7841_wholemount_possible.wlz
7841_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7841_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35954
Entity Detected:Ash1l, ash1 (absent, small, or homeotic)-like (Drosophila) ( MGI:2183158)
Sequence:sense strand is shown

>T35954
GAAGGCGCACTTTGTTTATACCAGAAAATAGCTTTCGGAAGTGACTCTCAAAAGGATGAGAACCGCGAGC
GAGCACCTGGGATCCAACGGCACCATCAAGTCCAGCCTCTTGCTCTCTGGACATGGACTGGGGCAGAAAA
GTTCTGTTCAGGCAAGGGGGGTGGGGTCACGTTTCTGACATGGTACTTTCTAAAGCTCGGAGCTAGTGGA
AAGGGAAAGGAAGTAGGCTTGTCTGAGACGGGTGCGTTTACTTCTCTCTCTGGCTTCTCCTGAGGGTTAA
CAGAGACAGGAGGACCACACTTGGAGTAGCCAGAAGCTACTGGTACTTTCCAGCACTTCCAAAGGGAAGG
GTCCGGACCCAGCCTGCCCCGTTTATCTCTTAGCTCTAAGAGCTACGTCAGAGACCTAAACAGGTACAGT
AGCTGAGGCCTCTTTTAAGTTCCTTTAACGGGAAAGTTCTTTCTCCCTTCTTGCCCAACCAGGTCAAAGT
ACTAGAACATGAATCAGCTGCTCAGAGCACTTAGAACTGCTGCCACTCTCCCTACCTCTGTTCCCAGAGT
AGAGTCAGTAGAACCAGGGAGGCGGAGAGGCCCCCATGGGGTCCGAAGGGCAGTATTGGTAATT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96669. Forward Primer - name:096669_F_cDNA_Ash1l, sequence:GAAGGCGCACTTTGTTTATACC; Reverse Primer - name:096669_N_SP6_cDNA_Ash1l, sequence:AATTACCAATACTGCCCTTCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7843 same embryo
 EMAGE:7844 same embryo
 EMAGE:7842 same embryo
 EMAGE:7845 same embryo
 EurExpress:euxassay_007724 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS