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EMAGE:785

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:785 EMAGE:785 EMAGE:785 EMAGE:785 EMAGE:785
10.5-3.jpg 10.5-10.jpg 10.5-12.jpg 10.5-4.jpg 10.5-6.jpg
EMAGE:785 EMAGE:785
10.5-9.jpg Sense control 10.5dpc

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Data from image 10.5-3.jpg was used for the spatial mapping in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:785Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
785_wholemount_strong_3D_1.wlz
785_wholemount_moderate_3D_1.wlz
785_wholemount_weak_3D_1.wlz
785_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:785_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Crapb1 cloneA
Entity Detected:Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Sequence:sense strand is shown

>Crapb1 cloneA
TGGCCAACGATGAGCTAATCCTGACATTTGGCGCCGATGATGTGGTGTGCACAAGAATTTATGTCCGGGA
GTAAAGGTGGCCAGCTTGTTCCTGCTTCATGACCGGATGCGAGTTCCCCTGAGGAGTATGCCGTGGCCCC
ACACTGCCAGTGGGTCTTTACTCCACACACCTCTCCCCCATGAATATTAGGCAACCCCATTTTCCCCATG
ACATTGTTGTAGTGTCCTCCCCTCAGGCTCTTGTTGCCTTGTGTACCCTTGGTTTGGCATTTGCATGATT
GTACCAGTCATTAAACTGGTTGGCTGC
nt 435 - nt 741 of NM_013496.1
Notes:The probe used in this study by Bennetts & Wicking covers exon 4 and the entire 3'UTR. Vector - pGEMTeasy. Antisense - NcoI/Sp6; Sense - SalI/T7 Conc of probe used in hyb 0.5-1microgram/ml
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jennifer Bennetts & Carol Wicking.
Principal investigator:Carol Wicking, Institute for Molecular Bioscience, Institute for Molecular Bioscience University of Queensland St Lucia, Brisbane, Australia 4072
Submitted by:Jennifer Bennetts, Institute for Molecular Bioscience, Institute for Molecular Bioscience University of Queensland St Lucia, Brisbane, Australia 4072
Experiment type:non-screen
References:[ doi:10.1002/gene.10165] [ PMID:12533789] Fowles LF, Bennetts JS, Berkman JL, Williams E, Koopman P, Teasdale RD, Wicking C 2003 Genomic screen for genes involved in mammalian craniofacial development. Genesis (35):73-87
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE