EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7855

Adamts12 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 ( MGI:2146046)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007605. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007605_01	euxassay_007605_02	euxassay_007605_03	euxassay_007605_04

euxassay_007605_05	euxassay_007605_06	euxassay_007605_07	euxassay_007605_08	euxassay_007605_09

euxassay_007605_10	euxassay_007605_11	euxassay_007605_12	euxassay_007605_13	euxassay_007605_14

euxassay_007605_15	euxassay_007605_16	euxassay_007605_17	euxassay_007605_18	euxassay_007605_19

euxassay_007605_20	euxassay_007605_21	euxassay_007605_22	euxassay_007605_23	euxassay_007605_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7855_wholemount_strong.wlz

7855_wholemount_moderate.wlz

7855_wholemount_weak.wlz

7855_wholemount_possible.wlz

7855_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7855_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05 06 07 19 20 21 22 23 24

fibula

moderate

regional

moderate expression: see section 24 weak expression: see section 02 03

tibia

moderate

regional

moderate expression: see section 24 weak expression: see section 02 03 04

femur

weak

regional

weak expression: see section 05 23 24

submandibular gland primordium

moderate

regional

moderate expression: see section 04 05 06 07 08 16 19 weak expression: see section 18 20

diencephalon meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 08 09 17 18 19

telencephalon meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

midbrain meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 07 08 09 17 18 19 20 21

spinal cord floor plate

weak

regional

weak expression: see section 13 14

tongue muscle

weak

regional

weak expression: see section 10 11 13 14

lower lip

moderate

regional

moderate expression: see section 09 10 11 12 weak expression: see section 06 07 08 13 14 15 16

mandible

moderate

regional

moderate expression: see section 02 03 04 05 06 07 16 21 weak expression: see section 08 09 10 14 15 17 18 19 20 22

upper lip

moderate

regional

moderate expression: see section 04 09 10 weak expression: see section 05 07 08 13 14 15 16

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 16 20 21 weak expression: see section 08 09 10 14 15 17 18 19

clavicle

weak

regional

weak expression: see section 08 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35885
Entity Detected:	Adamts12, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 ( MGI:2146046)
Sequence:	sense strand is shown >T35885 CAGTAGGCAACTGGAGTGAGTGCTCTGTTTCCTGTGGTGGTGGAGTGCGGATTCGCAGTGTCACATGTGC CAAGAACCTTAATGAGCCTTGTGACAAGACCCGGAAACCCAACAGTCGAGCTCTGTGCGGCCTCCAGCAG TGCCCATTTAGCCGTAGAGTTCTGAAACCCCATAAAGATATAGCTCCCAGTGGGAAAAATCAATCGACAG CAGAACATGACCCCTTCAAGCCCATCCCAGCACCAACATCCAGGCCCACGCCACTGTCCACACCCACAGT GCCTGAGTCTATGAGCACAAGTACTCCCACAATCAACAGCCTTGGCTCCACCATAGCATCCCAAGAAGAC GCAAATGGGATGGGATGGCAGAATAATTCAACCCAAGCTGAAGAGGGCTCCCACTTCCCTACTTCTTCTG GAAGCACTTCCCAGGTCCCCGTGACTTCCTGGTCTTTGAGTATCCAACCTGATGATGAGAATGTTTCCAG CTCAGCTATAGGTCCTACTTCAGAGGGTGACTTTTGGGCAACCACAACAAGTGATTCTGGCTTATCATCT AGCGATGCTATGACTTGGCAGGTAACTCCATTTTATAGCACCATGACCACAGATCCAGAAGTGGAGATAC ATAGTGGTTCAGGGGAAGACAGTGACCAGCCTTTGAACAAGGACAAAAGCAACTCTGTGATATGGAATAA GATCGGGGTACCTGAACATGATGCTCCCATGGAGACGGATGCAGAACTACCACTTGGACCTCCACCAACA TCTTATATGGGGGAAGAGCCCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93659. Forward Primer - name:093659_F_cDNA_Adamts12, sequence:CAGTAGGCAACTGGAGTGAGTG; Reverse Primer - name:093659_N_SP6_cDNA_Adamts12, sequence:GAGGGCTCTTCCCCCATATAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7854 same embryo
	EMAGE:7859 same embryo
	EMAGE:7856 same embryo
	EMAGE:7858 same embryo
	EMAGE:7857 same embryo
	EurExpress:euxassay_007605 same experiment
	MGI:4822982 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source