Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7857

Adamts20 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20 ( MGI:2660628)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857
"Pseudo-wholemount" of euxassay_007608. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007608_01 euxassay_007608_02 euxassay_007608_03 euxassay_007608_04
EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857
euxassay_007608_05 euxassay_007608_06 euxassay_007608_07 euxassay_007608_08 euxassay_007608_09
EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857
euxassay_007608_10 euxassay_007608_11 euxassay_007608_12 euxassay_007608_13 euxassay_007608_14
EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857
euxassay_007608_15 euxassay_007608_16 euxassay_007608_17 euxassay_007608_18 euxassay_007608_19
EMAGE:7857 EMAGE:7857 EMAGE:7857 EMAGE:7857
euxassay_007608_20 euxassay_007608_21 euxassay_007608_22 euxassay_007608_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7857Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7857_wholemount_strong.wlz
7857_wholemount_moderate.wlz
7857_wholemount_weak.wlz
7857_wholemount_possible.wlz
7857_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7857_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tongue muscle
weak weak
regionalweak expression: see section 10 11 12
lower jaw incisor
weak weak
regionalweak expression: see section 09 10 13 14
upper jaw incisor
weak weak
regionalweak expression: see section 09 10 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35888
Entity Detected:Adamts20, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20 ( MGI:2660628)
Sequence:sense strand is shown

>T35888
CTACTTCCTGTGATGGGTCCTCCAAGCCCCCAGAGTCAAGGCACTGTGGCTCCGGACCTTGTCCGCACTG
GAATTATGGGGACTGGGGAGAATGCACACAAACCTGTGGTGGAGGGGTAAAATCAAGATTTGTGATCTGT
CAGTTTCCCAATGGCCAAATGACACAGGAACACAGCTGTGAACTGCCCAAGCCACCTAGCATGATGCAGT
GTCACCTGCACGCCTGCCCTGAAGATGTGTCCTGGTATCGGGGACCATGGAAGTCGTGCTCAGCTTCCTG
TGGTAAAGGTGTAAAGTACCGGGAGGTTCTTTGTATTGATCAATTCCAAAGGAAATTAGAAGAAAAATAT
TGCAGTCATTTGCATAAACCTCGAACTCACAAAGCTTGCAGATCGGGACGATGCCCTTCATGGAAGGCCA
ATAAATGGAAGGAGTGCTCTGTGACCTGTGGGTCAGGGGTGCAGCAGAGGGAGGTGTACTGCAGGCTGAG
AGGCACTGGTCGAGTGTCTGAAGACATGTGTGATCCATCCACCCGGCCTCAGGGGCAGCGGCAGTGCTGG
CGTCAGGACTGCATGAGGTACCAGTGGACGACAGGAGACTGGCTAGATTGTTCAACATCGTGTAAGAAAA
AAGAGACCTATCGGCTGGTGAAATGTGTGAATGAGCAAAACGTACAAGCCAATGAAAGTCTCTGTGACCC
TTTAACGAAACCGCTTTCAATCAAAAAATGCAGGAACCCTCACTGCAAGTACTCGGTGGTTACAGGAGAC
TCGTCGCAGTGTGCAGGTAACTGTGGGTTCACTTCCCCACAGAAGATTACATACTGCACTAAGATCCAGT
CCTCTAAGAAGCATACGTTCCATCAGCTTCGGCCTGTCGTCTATGGGGAATGTCCGGTGATACCGTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95629. Forward Primer - name:095629_F_cDNA_Adamts20, sequence:CTACTTCCTGTGATGGGTCCTC; Reverse Primer - name:095629_N_SP6_cDNA_Adamts20, sequence:GAGACGGTATCACCGGACATT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7854 same embryo
 EMAGE:7859 same embryo
 EMAGE:7855 same embryo
 EMAGE:7856 same embryo
 EMAGE:7858 same embryo
 EurExpress:euxassay_007608 same experiment
 MGI:4822988 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS