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EMAGE:7863

Epha10 Eph receptor A10 ( MGI:3586824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863
"Pseudo-wholemount" of euxassay_010117. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010117_01 euxassay_010117_02 euxassay_010117_03 euxassay_010117_04
EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863
euxassay_010117_05 euxassay_010117_06 euxassay_010117_07 euxassay_010117_08 euxassay_010117_09
EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863
euxassay_010117_10 euxassay_010117_11 euxassay_010117_12 euxassay_010117_13 euxassay_010117_14
EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863
euxassay_010117_15 euxassay_010117_16 euxassay_010117_17 euxassay_010117_18 euxassay_010117_19
EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863 EMAGE:7863
euxassay_010117_20 euxassay_010117_21 euxassay_010117_22 euxassay_010117_23 euxassay_010117_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7863Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7863_wholemount_strong.wlz
7863_wholemount_moderate.wlz
7863_wholemount_weak.wlz
7863_wholemount_possible.wlz
7863_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7863_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38162
Entity Detected:Epha10, Eph receptor A10 ( MGI:3586824)
Sequence:sense strand is shown

>T38162
GACTGCAGTAGCATACCAGGTGCCACGGGCACCTGCAAAGAGACCTTCAACGCATACTACCTAGAAACCG
AGACCGACCTGGGCCGAGGGCGTCCCCGCCTAGGAGGCAACCGACCCCGCAAGATTGACACCATCTCAGC
TGATGAGAGCTTCACGCAGGGTGACCTGGGGGAGCGAAAGATGAAGCTGAACACAGAGGTGCGTGAGATC
GGCCCCCTCAGCCGGCAAGGCTTCCACCTGGCCTTCCAGGACGTGGGTGCGTGCGTGGCGTTGGTCTCGG
TGCGTGTCTACTACAAGCAGTGCCGCGCCACAGTGCGGGGCCTGGCAGCCTTCCCCGCCACCGCAGCTGA
GAGCGCCTTCTCCACGCTGGTGGAAGTGGCTGGAACGTGCGTGGCCCACTCGGAAGGGGAGCCCAGCAGC
CCTCCCCGCATGCACTGTGGTGCCGATGGCGAGTGGCTGGTGCCTGTGGGCCGTTGCAGCTGCAGTGCAG
GATTCCAGGAACATGGTGACATCTGTGAAGGTATCCAGGGCCCCAGAGGGATGGGATAGGGACATATCGT
GCAGATGGCAGGATAGAGCGTGCTCTGGGTGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105327. Forward Primer - name:105327_F_cDNA_A330090H18, sequence:GACTGCAGTAGCATACCAGGTG; Reverse Primer - name:105327_N_SP6_cDNA_A330090H18, sequence:TCACCCAGAGCACGCTCTATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7866 same embryo
 EMAGE:7864 same embryo
 EMAGE:7865 same embryo
 EMAGE:7867 same embryo
 EMAGE:7862 same embryo
 EurExpress:euxassay_010117 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS