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EMAGE:787

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:787 EMAGE:787 EMAGE:787 EMAGE:787 EMAGE:787
12.5d_2.jpg 12.5d_3.jpg 12.5d_4.jpg 12.5d_5.jpg 12.5d_6.jpg

Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regional
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Crapb1 cloneA
Entity Detected:Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Sequence:sense strand is shown

>Crapb1 cloneA
TGGCCAACGATGAGCTAATCCTGACATTTGGCGCCGATGATGTGGTGTGCACAAGAATTTATGTCCGGGA
GTAAAGGTGGCCAGCTTGTTCCTGCTTCATGACCGGATGCGAGTTCCCCTGAGGAGTATGCCGTGGCCCC
ACACTGCCAGTGGGTCTTTACTCCACACACCTCTCCCCCATGAATATTAGGCAACCCCATTTTCCCCATG
ACATTGTTGTAGTGTCCTCCCCTCAGGCTCTTGTTGCCTTGTGTACCCTTGGTTTGGCATTTGCATGATT
GTACCAGTCATTAAACTGGTTGGCTGC
nt 435 - nt 741 of NM_013496.1
Notes:The probe used in this study by Bennetts & Wicking covers exon 4 and the entire 3'UTR. Vector - pGEMTeasy. Antisense - NcoI/Sp6; Sense - SalI/T7 Conc of probe used in hyb 0.5-1microgram/ml
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jennifer Bennetts & Carol Wicking.
Principal investigator:Carol Wicking, Institute for Molecular Bioscience, Institute for Molecular Bioscience University of Queensland St Lucia, Brisbane, Australia 4072
Submitted by:Jennifer Bennetts, Institute for Molecular Bioscience, Institute for Molecular Bioscience University of Queensland St Lucia, Brisbane, Australia 4072
Experiment type:non-screen
References:[ doi:10.1002/gene.10165] [ PMID:12533789] Fowles LF, Bennetts JS, Berkman JL, Williams E, Koopman P, Teasdale RD, Wicking C 2003 Genomic screen for genes involved in mammalian craniofacial development. Genesis (35):73-87
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE