EMAGE mouse gene expression spatial database

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EMAGE:7887

Cblb Casitas B-lineage lymphoma b ( MGI:2146430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012817. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012817_01	euxassay_012817_02	euxassay_012817_03	euxassay_012817_04

euxassay_012817_05	euxassay_012817_06	euxassay_012817_07	euxassay_012817_08	euxassay_012817_09

euxassay_012817_10	euxassay_012817_11	euxassay_012817_12	euxassay_012817_13	euxassay_012817_14

euxassay_012817_15	euxassay_012817_16	euxassay_012817_17	euxassay_012817_18	euxassay_012817_19

euxassay_012817_20	euxassay_012817_21	euxassay_012817_22	euxassay_012817_23	euxassay_012817_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7887_wholemount_strong.wlz

7887_wholemount_moderate.wlz

7887_wholemount_weak.wlz

7887_wholemount_possible.wlz

7887_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7887_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

weak

regional

weak expression: see section 11

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 11 14

cerebral cortex marginal layer

strong

regional

strong expression: see section 03 04 05 06 21 22 moderate expression: see section 02 07 08 17 18 19 20 weak expression: see section 01 09 10 11 15 16 23

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 12 13 weak expression: see section 09

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 05 06 07 11 14 17 21 weak expression: see section 04 08 09 10 12 15 16 18 19 20

midbrain mantle layer

moderate

regional

moderate expression: see section 11 17 weak expression: see section 08 09 10 12 13 14 15 16 18

extrinsic ocular muscle

weak

regional

weak expression: see section 04 05 18 19 20

lower jaw incisor

weak

regional

weak expression: see section 09 10 13 14

upper jaw incisor

weak

regional

weak expression: see section 09 10 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38193
Entity Detected:	Cblb, Casitas B-lineage lymphoma b ( MGI:2146430)
Sequence:	sense strand is shown >T38193 GTGAACCTACACCTCACGATCATATAAAAGTTACACAGGAGCAGTATGAACTGTATTGTGAAATGGGCTC CACTTTTCAGCTCTGCAAGATCTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGCGGGCATCTG ATGTGCACTTCGTGCCTTACCGCGTGGCAGGAGTCTGATGGCCAAGGCTGCCCCTTCTGTCGCTGTGAGA TAAAAGGAACGGAGCCCATCATTGTGGACCCCTTCGACCCCAGAGATGAAGGCTCCAGGTGCTGCAGCAT CATTGACCCTTTCAGCATCCCCATGCTTGACTTGGACGATGACGATGATCGAGAGGAGTCTTTGATGATG AACAGGCTGGCGAGTGTTCGAAAGTGCACGGACAGGCAGAACTCACCAGTCACATCGCCAGGCTCCTCAC CCCTTGCCCAGAGAAGAAAGCCTCAGCCAGACCCTCTCCAGATCCCCCACCTCAGCCTGCCACCAGTGCC TCCCCGCCTAGATCTCATTCAGAAAGGCATCGTGCGCTCTCCGTGTGGCAGCCCCACAGGCTCGCCAAAG TCTTCTCCATGCATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104117. Forward Primer - name:104117_F_cDNA_Cblb, sequence:GTGAACCTACACCTCACGATCA; Reverse Primer - name:104117_N_SP6_cDNA_Cblb, sequence:CATGCATGGAGAAGACTTTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7883 same embryo
	EMAGE:7886 same embryo
	EMAGE:7884 same embryo
	EMAGE:7885 same embryo
	EMAGE:7882 same embryo
	EurExpress:euxassay_012817 same experiment
	MGI:4823651 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source