EMAGE mouse gene expression spatial database

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EMAGE:7893

Urm1 ubiquitin related modifier 1 homolog (S. cerevisiae) ( MGI:1915455)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012803. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012803_01	euxassay_012803_02	euxassay_012803_03	euxassay_012803_04

euxassay_012803_05	euxassay_012803_06	euxassay_012803_07	euxassay_012803_08	euxassay_012803_09

euxassay_012803_10	euxassay_012803_11	euxassay_012803_12	euxassay_012803_13	euxassay_012803_14

euxassay_012803_15	euxassay_012803_16	euxassay_012803_17	euxassay_012803_18	euxassay_012803_19

euxassay_012803_20	euxassay_012803_21	euxassay_012803_22	euxassay_012803_23	euxassay_012803_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7893_wholemount_strong.wlz

7893_wholemount_moderate.wlz

7893_wholemount_weak.wlz

7893_wholemount_possible.wlz

7893_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7893_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 17 weak expression: see section 09 10 16

pons mantle layer

weak

regional

weak expression: see section 08 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 20 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 09 17 20 21 22 weak expression: see section 07 08 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 08 18 20

ventral grey horn

moderate

regional

moderate expression: see section 15 weak expression: see section 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 12 17 weak expression: see section 06 07 13 14 15 16

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

mandible

weak

regional

weak expression: see section 05 06 07 08 09 10 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38207
Entity Detected:	Urm1, ubiquitin related modifier 1 homolog (S. cerevisiae) ( MGI:1915455)
Sequence:	sense strand is shown >T38207 ACTGATGTCATTATGGAACCCCAGCTGGGGTCCCCTATTCAGTGCTCCCCTCCCTCCTCCACCCAGCAGC CGCTCTCCCCCCCACCCCCTTCTCCATCCTTTGACCCTGGCTGGCCTCCCCCCCACATAGGCCACCAGAT GGGCTCCTGAGATACTCTCCAGGCTGCCTACAACTCATTCTGTTGCGGGTAGAGAATGGAAGGGAGCAGC CTAACCGTAGGCAGAGGGGGCGGGGCCTCAGCATGTGTAACTCCAGGCTTCCTGAAACAAGTGGGATTTG GGTGTGCAGCACCTAGTGGGCAGAAGGCTGTGTGTGTCCCTTTCCTCTATCTTCTGGAGACTTCATGCCT GCAGCAGCTGGCCTCGGGCAAATAAAAAGACTAAGTGACTAGGTTGTTTACTAGCCTTGCTTCACCCTTA AAACTGGTGGGCCCTGTACTCTCTGGGTCTGTGGGTTCTAATACAAATGCCCTGTTCTGAGCCAAGGGTT CAGAGATGGCCTTCACCTGAAAGGTAAAAACATGAACTGTTCCAAAGAGAGGGTGGATGAGTAGGACGGT GTGGATGGGGAGGGAGTGTAAGCCCGTGATGACCAGCCCACATTCTAAGCCAGGATTTCTCACCAACCAG TCCAAGCTAGGCTTTGTTCTGCCAACTGCAGTCACCAGTCCCAGAAGGGGTGGCCTAAGTAAGCCTGCTG AGTCTCATTACTTCCACAACCTGCAGAAAAAGCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 146321. Forward Primer - name:146321_F_cDNA_2900073H19Rik, sequence:ACTGATGTCATTATGGAACCCC; Reverse Primer - name:146321_N_SP6_cDNA_2900073H19Rik, sequence:AAGCTTTTTCTGCAGGTTGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7894 same embryo
	EMAGE:7896 same embryo
	EMAGE:7895 same embryo
	EurExpress:euxassay_012803 same experiment
	MGI:4829119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source