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EMAGE:794

Cdkn1c cyclin-dependent kinase inhibitor 1C (P57) ( MGI:104564)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:794 EMAGE:794
92S 8.5 1 92S 8.5 4

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:794Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
794_wholemount_strong_3D_1.wlz
794_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:794_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdkn1c cloneA
Entity Detected:Cdkn1c, cyclin-dependent kinase inhibitor 1C (P57) ( MGI:104564)
Sequence:sense strand is shown

>Cdkn1c cloneA
A
nt 1509 - nt 1744 of NM_009876.1
Notes:The Cdkn1c (p57Kip2) probe used in this study by Fowles & Wicking is 235nt long and is found within the 3' UTR. Vector - pGEMTEasy; Antisense - SalI/T7; Sense - NcoI/Sp6.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lindsay Fowles & Carol Wicking
Principal investigator:Carol Wicking, Institute for Molecular Bioscience, Institute for Molecular Bioscience University of Queensland St Lucia, Brisbane, Australia 4072
Submitted by:Lindsay Fowles, Institute for Molecular Bioscience, Institute for Molecular Bioscience University of Queensland St Lucia, Brisbane, Australia 4072
Experiment type:non-screen
References:[ doi:10.1002/gene.10165] [ PMID:12533789] Fowles LF, Bennetts JS, Berkman JL, Williams E, Koopman P, Teasdale RD, Wicking C 2003 Genomic screen for genes involved in mammalian craniofacial development. Genesis (35):73-87
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE