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EMAGE:7973

Klhdc5 kelch domain containing 5 ( MGI:2444786)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973
"Pseudo-wholemount" of euxassay_013105. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013105_01 euxassay_013105_02 euxassay_013105_03 euxassay_013105_04
EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973
euxassay_013105_05 euxassay_013105_06 euxassay_013105_07 euxassay_013105_08 euxassay_013105_09
EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973
euxassay_013105_10 euxassay_013105_11 euxassay_013105_12 euxassay_013105_13 euxassay_013105_14
EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973
euxassay_013105_15 euxassay_013105_16 euxassay_013105_17 euxassay_013105_18 euxassay_013105_19
EMAGE:7973 EMAGE:7973 EMAGE:7973 EMAGE:7973
euxassay_013105_20 euxassay_013105_21 euxassay_013105_22 euxassay_013105_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7973Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7973_wholemount_strong.wlz
7973_wholemount_moderate.wlz
7973_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7973_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
spottedmoderate expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38655
Entity Detected:Klhdc5, kelch domain containing 5 ( MGI:2444786)
Sequence:sense strand is shown

>T38655
CTGTGTAGCAGGAAGTGTCTGGGAGTACCCCCAAGGGAGCTTCCTGTCAAGTGTGCACTCCTACTCCGGG
AGGCTTCTCCCCTTGGCACCAGAAAGGTTCTGAGATGATTGTGTGAGAATCTCTAACTTCTGCGATCTGA
GCTAGGACATATTGTCAAAGAACCTACAAGGTGGGGGATGCTGGCAAGAAACGCATACGGCTTAGGATTA
AGCATGAAACCAAAGAGACCTTGACCCTGCTGTGTTCTGTTGTTTCTAACGCCAAGAACCCAAGCAAACT
TAAATGCCTTAGGTTTCCGTTCAAGCAGAGGGAAGGCGAGGATACTGTAGCTCTATACTGGGAGAACAGA
GGAAAATGTTTCTTTTTAATAAATAGTTGTTTATAGACTATTAGAGGAGCAGTCCTCATAATTCCAGGGA
AAGTTATAAGCCTGTTTGGCCAAAGAGGTAAATAGAAGGGGCCCTGATGTCTTTGTCAAGGTGTTGGGTG
AGCTTAGCTTAGCACTGCTGACGTGAGGGGCTGCAGGACAAAGGGATCATGCTGAAGGCTGTCTTGACCT
TACCCAGGAGTACGGGGTCTGTGTGTACACTCGCTCTGTCAGGGTTTGCACTTAGGAGACCTGTCAATCA
GTGCTACCAGACATCCTGCCAGCCTACCAGAGTGAAGTGAATGCCAGTTCCTGCCCCTGCCTCCGAGATA
GTCAGTATGAAGGGAGCTGTGATCTCTGGAAGTAGTGAGCTACATCAATTCCATTCCTTGGTGACCAGAA
ACATTAAGTGTTGGTGTGAGACAGAGCCTAGCCTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108287. Forward Primer - name:108287_F_cDNA_LOC434103, sequence:CTGTGTAGCAGGAAGTGTCTGG; Reverse Primer - name:108287_N_SP6_cDNA_LOC434103, sequence:CCAGGCTAGGCTCTGTCTCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7972 same embryo
 EMAGE:7976 same embryo
 EMAGE:7975 same embryo
 EMAGE:7974 same embryo
 EurExpress:euxassay_013105 same experiment
 MGI:4825789 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS