EMAGE mouse gene expression spatial database

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EMAGE:7983

Plcl1 phospholipase C-like 1 ( MGI:3036262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013151. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013151_01	euxassay_013151_02	euxassay_013151_03	euxassay_013151_04

euxassay_013151_05	euxassay_013151_06	euxassay_013151_07	euxassay_013151_08	euxassay_013151_09

euxassay_013151_10	euxassay_013151_11	euxassay_013151_12	euxassay_013151_13	euxassay_013151_14

euxassay_013151_15	euxassay_013151_16	euxassay_013151_17	euxassay_013151_18	euxassay_013151_19

euxassay_013151_20	euxassay_013151_21	euxassay_013151_22	euxassay_013151_23	euxassay_013151_24

euxassay_013151_25	euxassay_013151_26	euxassay_013151_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7983_wholemount_strong.wlz

7983_wholemount_moderate.wlz

7983_wholemount_weak.wlz

7983_wholemount_possible.wlz

7983_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7983_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 05 06 07 21 22 23 24

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 12 13 16

telencephalon mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 18 19 20 21 22 23 24 25 26

olfactory cortex mantle layer

weak

regional

weak expression: see section 11 12 13 16 17

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 10 11 12 17 18

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 12 13 15 16 17 18

pons mantle layer

weak

regional

weak expression: see section 09 20 21

midbrain floor plate

weak

regional

weak expression: see section 14 15

ventral grey horn

moderate

regional

moderate expression: see section 15 weak expression: see section 13 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39526
Entity Detected:	Plcl1, phospholipase C-like 1 ( MGI:3036262)
Sequence:	sense strand is shown >T39526 AGAATCCTACCTCCCATCACCTGAAAAACTAAAAAATATGATTATTGTGAAAGGAAAGAAATTGCCTTCG GAATCAGATCTGTTAGAAGGAGAAGTGACCGACGAGGACGAAGAAGCCGAGATGTCGCGGAGGATGTCAG GGGATTACAACGGTGAGCAGAAACATATCTGGCTCTGCCGAGAGCTCTCTGACTTGGTATCCATCTGCAA ATCTGTTCAGCACAGGGATTTTGAACTGTCTATGAAAACCCAAAACTACTGGGAAATGTGTTCATTTAGT GAAACAGAGGCCAGCCGGATTGCCAATGAATACCCAGAGGACTTCGTGAATTATAACAAGAAGTTCTTAT CAAGGGTCTACCCTAGTGCCATGCGGATAGATTCCAGTAATTTGAACCCACAGGACTTCTGGAACTGTGG CTGTCAGATTGTGGCGATGAATTTTCAAACTCCCGGTCCAATGATGGACCTCCACACTGGCTGGTTTCTT CAAAATGGAGGATGTGGCTACGTCCTCAGGCCATCTATCATGCGAGATGAAGTTTCGTACTTCAGCGCAA ATACAAAGGGCATTGTCCCTGGCGTGTCTCCCCTGGTTCTTCACATCAAGATCATCAGTGGTCAGAACTT CCCAAAGCCCAAAGGAGCCTGTGCCAAAGGGGATGTCATAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75607. Forward Primer - name:075607_F_cDNA_Plcl1, sequence:AGAATCCTACCTCCCATCACCT; Reverse Primer - name:075607_N_SP6_cDNA_Plcl1, sequence:CTATGACATCCCCTTTGGCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7985 same embryo
	EMAGE:7988 same embryo
	EMAGE:7989 same embryo
	EMAGE:7984 same embryo
	EMAGE:7986 same embryo
	EMAGE:7987 same embryo
	EurExpress:euxassay_013151 same experiment
	MGI:4827257 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source