EMAGE mouse gene expression spatial database

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EMAGE:7995

D930030O05Rik RIKEN cDNA D930030O05 gene ( MGI:2443927)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016032. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016032_01	euxassay_016032_02	euxassay_016032_03	euxassay_016032_04

euxassay_016032_05	euxassay_016032_06	euxassay_016032_07	euxassay_016032_08	euxassay_016032_09

euxassay_016032_10	euxassay_016032_11	euxassay_016032_12	euxassay_016032_13	euxassay_016032_14

euxassay_016032_15	euxassay_016032_16	euxassay_016032_17	euxassay_016032_18	euxassay_016032_19

euxassay_016032_20	euxassay_016032_21	euxassay_016032_22	euxassay_016032_23	euxassay_016032_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7995_wholemount_strong.wlz

7995_wholemount_moderate.wlz

7995_wholemount_weak.wlz

7995_wholemount_possible.wlz

7995_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7995_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 05 06 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 moderate expression: see section 19

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 09 10 17 18 20 21 22 23 moderate expression: see section 04 07 08 19

vagus x ganglion

strong

regional

strong expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08

ventral grey horn

moderate

regional

moderate expression: see section 10 11 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 14 15 16 17 18 19

testis

weak

regional

weak expression: see section 05 17 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39697
Entity Detected:	D930030O05Rik, RIKEN cDNA D930030O05 gene ( MGI:2443927)
Sequence:	sense strand is shown >T39697 GGAGCTCAAGGTCATTCTCAGTCACACATAAAATACTAGGCCAGCCTGGGCTACCTGAGACCTTGTCTCC AGAAAAGAAAAAGGGGAAAGGAAAAAAAAAGAACTAATGTATTTTAAAGTTGGAGAACAGCCTTAGACCT GCAGTGGACTGATGTCTACTTTTCATTTTGTAAAATATTTTGAACATTTCTATTTATCATTCATAATGCT CTAAATAGAAGCAGAACACTGTAGCTCTCAGATCCTGGGACAGGCAGGTCATGAGGGCTCTGACTGTTTG GGGACAGTCTGTGACTCTTGATGTTTTAAATGTAGCAATTATGGCCTTGAAAAACTGTAACTCTAATATT GGCATTTGTTGGTCATCTCAGATGAACCCAAACTGACCATTGCTGTGTTTGGGTGAAACCTGATGCTTTT GCTTTTTTTTCTCTGTTTGCTTTTTTCTCTATCATAAAAGTATTTAATTATAAAAATAAGTGCTTTTCAG ATTTCCACCTTCAATATATTGACTGTGGGTTTCTGATGCAGCATCAGACTGGAGCAGCCTGTCCTTTGAG GCCCAGTGTTTCCATGGCCTGGAAGGACCCTCTCCTGCCGTCAGTAACTTCTGTAGCTTCTTTCCTCCTA GATGTTCTTCCCTGTCCACCAGTGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69592. Forward Primer - name:069592_F_cDNA_D930030O05Rik, sequence:GGAGCTCAAGGTCATTCTCAGT; Reverse Primer - name:069592_N_SP6_cDNA_D930030O05Rik, sequence:GTCACTGGTGGACAGGGAAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7997 same embryo
	EMAGE:7998 same embryo
	EMAGE:7999 same embryo
	EMAGE:7994 same embryo
	EMAGE:7996 same embryo
	EurExpress:euxassay_016032 same experiment
	MGI:4824214 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source