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EMAGE:8015

Gtf3c3 general transcription factor IIIC, polypeptide 3 ( MGI:2138383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015
"Pseudo-wholemount" of euxassay_015986. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015986_01 euxassay_015986_02 euxassay_015986_03 euxassay_015986_04
EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015
euxassay_015986_05 euxassay_015986_06 euxassay_015986_07 euxassay_015986_08 euxassay_015986_09
EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015
euxassay_015986_10 euxassay_015986_11 euxassay_015986_12 euxassay_015986_13 euxassay_015986_14
EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015 EMAGE:8015
euxassay_015986_15 euxassay_015986_16 euxassay_015986_17 euxassay_015986_18 euxassay_015986_19
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euxassay_015986_20 euxassay_015986_21 euxassay_015986_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8015Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8015_wholemount_strong.wlz
8015_wholemount_moderate.wlz
8015_wholemount_weak.wlz
8015_wholemount_possible.wlz
8015_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8015_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
testis
weak weak
regionalweak expression: see section 04 05 06 07 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39525
Entity Detected:Gtf3c3, general transcription factor IIIC, polypeptide 3 ( MGI:2138383)
Sequence:sense strand is shown

>T39525
TGGCATCTCAGAAGTATGTGCTGAAGAGACATGCTCTGACTGTGCAGGGCTTTTCCTTCCTTAATCGATA
CCTCAGCATCCGTGGACCTTGCCAGGAATCTTTCTACAATTTGGGCCGTGGCCTTCATCAGCTGGGGCTG
ACACACCTTGCAATCCACTATTACCAGAAGGCTCTGGCACTCCCTCCACTTGTGGTCGAGGGTATAGAAG
TTGACCAGTTAGATTTACGAAGAGATATTGCCTACAACATGTCTCTCATTTATCAGAGCAGTGGGAACAC
TGCAATGGCTCAAAAGCTTTTGTATACCTACTGTGTCATATAAGCAGTGTAATTTATAAGACGATGTATG
GACCAGTATCTTCTAACGGGACCTCATTGTTCTTGATGATAAAAATGGTACTGTCATCACTGCTTGCTAC
CATTACAACTGCTTTGAAGCAGGGGATGTAGCTCAGCTGTAGACTGCCTGCTTGCTTCATATGTACAAGG
CCCCAGATTCAAGCCTTTGGAAAATTGAAACACAGCTTTTACTCTGGAATATATGCAAAGTTGCTATTCC
TATGAGAATGTACATAATATTCTTTGTTGTTAGCATTCTACAATAAAATTATTCCAAACACTGAAATGGA
CAATATTGCCTTCTGAATCAGTTGGAATGGAACATACTATTTTTCTACTTAATATCTGCTAAACTCCTGT
GAACC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 106152. Forward Primer - name:106152_F_cDNA_LOC227109, sequence:TGGCATCTCAGAAGTATGTGCT; Reverse Primer - name:106152_N_SP6_cDNA_LOC227109, sequence:GGTTCACAGGAGTTTAGCAGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8014 same embryo
 EMAGE:8017 same embryo
 EMAGE:8016 same embryo
 EMAGE:8013 same embryo
 EMAGE:8012 same embryo
 EurExpress:euxassay_015986 same experiment
 MGI:4825282 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS