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EMAGE:8025

Fam81b family with sequence similarity 81, member B ( MGI:2685122)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025
"Pseudo-wholemount" of euxassay_015991. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015991_01 euxassay_015991_02 euxassay_015991_03 euxassay_015991_04
EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025
euxassay_015991_05 euxassay_015991_06 euxassay_015991_07 euxassay_015991_08 euxassay_015991_09
EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025
euxassay_015991_10 euxassay_015991_11 euxassay_015991_12 euxassay_015991_13 euxassay_015991_14
EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025
euxassay_015991_15 euxassay_015991_16 euxassay_015991_17 euxassay_015991_18 euxassay_015991_19
EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025 EMAGE:8025
euxassay_015991_20 euxassay_015991_21 euxassay_015991_22 euxassay_015991_23 euxassay_015991_24
EMAGE:8025 EMAGE:8025
euxassay_015991_25 euxassay_015991_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8025Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8025_wholemount_strong.wlz
8025_wholemount_moderate.wlz
8025_wholemount_possible.wlz
8025_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8025_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
weak weak
regionalweak expression: see section 07 08 09 10 11 12 14 16 17 18 19 20
metencephalon part of 4th ventricle choroid plexus
weak weak
regionalweak expression: see section 06 07 08 10 11 12 14 15 16 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39575
Entity Detected:Fam81b, family with sequence similarity 81, member B ( MGI:2685122)
Sequence:sense strand is shown

>T39575
TGAGTATGTTGACAAGGGTTGCCCCTTTCTTCCTGCCATGCCAAGCAACCAAACAAGTCAGCTAGAAGAC
AGGCTGAGTCACCAGGAGCGCACCGTAGCCTTCCTCCTTGAACAGGCTTTCCGAATCAAGGAGGACATCT
CTGCTTGCCTCCACGGAAGCCAGGGGTTTCGAAAAGAAGAATCTCTTGCCAGGAAGTTACTAGAAAGCCA
CATACAGACCATCACCAGCATCGTCAAGAAACTCAACCAAAACATTGAGCTCCTAGAAGACCAAATAAGA
ATCCGAGACCAGGTGACCACAGGAACTAACTTTGCAGTGCAGGATCTAAACATCAAACACATACAAGGAG
TTGGAGACCTTCGCGGAAGAGTAGCCAGATGTGATTCAAGCATTGTGAAGCTTTCCGGAGACATCCAGTT
CATCAGACATGAGTGCCGACAGATGGAGAAATCAGTGCAAGAACTTGCTTCTGCCCTAGAAACCATTTCT
AAGAACTTGGATGTGAAGGTAATGCAGCTTTTAGGAAAGATAGAGGCCTCTGCCTCTGAGCACACCTCAA
ATTTAAAGATGGTCCGAGGAGATTATCAGCATGAAATGAACCTCTTAGAATTCAAATTTAACTCACTTTC
TAAAAATATATATGAAGAAGTTGAAACCAATCAAAAATGGACCGAAAACCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84441. Forward Primer - name:084441_F_cDNA_LOC238726, sequence:TGAGTATGTTGACAAGGGTTGC; Reverse Primer - name:084441_N_SP6_cDNA_LOC238726, sequence:CTGGTTTTCGGTCCATTTTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8027 same embryo
 EMAGE:8028 same embryo
 EMAGE:8026 same embryo
 EMAGE:8029 same embryo
 EMAGE:8024 same embryo
 EurExpress:euxassay_015991 same experiment
 MGI:4824761 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS