EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8040

Ttc6 tetratricopeptide repeat domain 6 ( MGI:2684915)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_015977_01	euxassay_015977_02	euxassay_015977_03	euxassay_015977_04	euxassay_015977_05

euxassay_015977_06	euxassay_015977_07	euxassay_015977_08	euxassay_015977_09	euxassay_015977_10

euxassay_015977_11	euxassay_015977_12	euxassay_015977_13	euxassay_015977_14	euxassay_015977_15

euxassay_015977_16	euxassay_015977_17	euxassay_015977_18	euxassay_015977_19	euxassay_015977_20

euxassay_015977_21	euxassay_015977_22	euxassay_015977_23	euxassay_015977_24	euxassay_015977_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8040_wholemount_strong.wlz

8040_wholemount_moderate.wlz

8040_wholemount_weak.wlz

8040_wholemount_possible.wlz

8040_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8040_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

weak

regional

weak expression: see section 12 13

metencephalon floor plate

weak

regional

weak expression: see section 12

midbrain floor plate

moderate

regional

moderate expression: see section 12

midbrain roof plate

weak

regional

weak expression: see section 13

trachea

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39492
Entity Detected:	Ttc6, tetratricopeptide repeat domain 6 ( MGI:2684915)
Sequence:	sense strand is shown >T39492 ACTGTAACGAGGCCATCAAACTTTACCCTGAGAGTGTGCGTGCCTACATCTGCAGAGGAGTTCTGAAATA CTACAACAGAACTTACAAGCTAGGTATTACAGATTTATCTACAGCTATCAAGATGGACAAGAACAATTAC ACAGCATATTATAACAGAGCCCTGTGTTACACCAAGATAGGGGAGCATCAGATGGCTTTAAGAGATTATG GAATTGTGCTGCTTCTTGATGCTGGAGAAAACATAGCATTAAATACCCTCATTAACCGTGGACTCATCTA TACTGAACTGAAGCAGTATGGCTTTGCACTAGAGGATTTTAAACAAGCCGCACTGATGAGCGGGACTAGT GTGAGCCTTTGTCAAGCTACTGCCACGTGCCACCACAGAATTAAAGAGTTTGAAGGAGCTGTTGAATTCT TTACCAGGGCTATCAAAATCAACCCACATTACGTGGATGCTTATATTGGACGGGGGAATTCTTACATGGA GTATAGTCAGGAAGATGCTATGATACAAGCCCAGAAGGACTTTCTGAAAGCACTTCATCTTGACCCATCA TGTTTAAAAGCCAGAATTAGTCTAGGCTATAATTTGCAGGCTCAAGGAAAATTCCAGAAAGCTTGGATAC ACTTTACTGTTGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83676. Forward Primer - name:083676_F_cDNA_LOC217602, sequence:ACTGTAACGAGGCCATCAAACT; Reverse Primer - name:083676_N_SP6_cDNA_LOC217602, sequence:GCCAACAGTAAAGTGTATCCAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8037 same embryo
	EMAGE:8041 same embryo
	EMAGE:8038 same embryo
	EMAGE:8036 same embryo
	EMAGE:8039 same embryo
	EurExpress:euxassay_015977 same experiment
	MGI:4828992 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source