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EMAGE:808

T brachyury ( MGI:98472)
TS10 (MS Downs & Davies)
in situ hybridisation

Data Images
EMAGE:808
Figure 3A of Lowe et al., 2001 [PMID:11311163] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is defined as a mid streak (MS) stage embryo (see Downs & Davies [PMID:8269852] for staging criteria).
Expression Pattern Description
Spatial Annotation:
EMAGE:808Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
808_wholemount_strong_3D_1.wlz
808_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:808_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
primitive streak
detected detected
homogeneousExpression is detected throughout streak.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2158745
Entity Detected:T, brachyury ( MGI:98472)
Notes:The T probe used in this study by Lowe et al., 2001 [PMID:11311163] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:MS Downs & Davies
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BCIP
General Information
Authors:Lowe et al., 2001 [PMID:11311163] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11311163] Lowe LA, Yamada S, Kuehn MR 2001 Genetic dissection of nodal function in patterning the mouse embryo. Development (128):1831-43
 [ PMID:8269852] Downs KM, Davies T 1993 Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope. Development (118):1255-66
Links:MGI:2159412 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI