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EMAGE:81

Six3 sine oculis-related homeobox 3 homolog (Drosophila) ( MGI:102764)
TS11 (7.75 dpc)
in situ hybridisation

Data Images
EMAGE:81
Figure 2A, Rhinn et al., 1998 [PMID:9449667] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:81Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
81_wholemount_strong_3D_1.wlz
81_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:81_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalSix3 is expressed in the presumptive forebrain region in wild-type embryos
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1315062
Entity Detected:Six3, sine oculis-related homeobox 3 homolog (Drosophila) ( MGI:102764)
Notes:Probe used in this study by Rhinn et al., 1998 [PMID:9449667] is indicated as being described by Oliver et al., 1995 [PMID:8575305] . Oliver et al., 1995 describe a number of "Bluescript KS II+ subclones containing different coding regions of Six3" as in situ hybridisation probes.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:(CD1 x 129/Sv)F1
Age:7.75 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Rhinn et al., 1998 [PMID:9449667] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8575305] Oliver G, Mailhos A, Wehr R, Copeland NG, Jenkins NA, Gruss P 1995 Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development. Development (121):4045-55
 [ PMID:9449667] Rhinn M, Dierich A, Shawlot W, Behringer RR, Le Meur M, Ang SL 1998 Sequential roles for Otx2 in visceral endoderm and neuroectoderm for forebrain and midbrain induction and specification. Development (125):845-56
Links:MGI:1315073 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI