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EMAGE:8106

5330438I03Rik RIKEN cDNA 5330438I03 gene ( MGI:2685831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106
"Pseudo-wholemount" of euxassay_016180. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016180_01 euxassay_016180_02 euxassay_016180_03 euxassay_016180_04
EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106
euxassay_016180_05 euxassay_016180_06 euxassay_016180_07 euxassay_016180_08 euxassay_016180_09
EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106
euxassay_016180_10 euxassay_016180_11 euxassay_016180_12 euxassay_016180_13 euxassay_016180_14
EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106
euxassay_016180_15 euxassay_016180_16 euxassay_016180_17 euxassay_016180_18 euxassay_016180_19
EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106 EMAGE:8106
euxassay_016180_20 euxassay_016180_21 euxassay_016180_22 euxassay_016180_23 euxassay_016180_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8106Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8106_wholemount_strong.wlz
8106_wholemount_moderate.wlz
8106_wholemount_weak.wlz
8106_wholemount_possible.wlz
8106_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8106_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
moderate moderate
single cellmoderate expression: see section 10 weak expression: see section 11 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45437
Entity Detected:5330438I03Rik, RIKEN cDNA 5330438I03 gene ( MGI:2685831)
Sequence:sense strand is shown

>T45437
ACATGTTTTACTTCACCCACCCTCACAACCACAGCAAACAGCAACACACAGCTCTGGTCCCAGGAGACCA
GGAATAATTGTTAACATAGAAAGGATTGTATCTCTCTCCGGGGCAGGGACCAAGTTGCCTATTAAGTCAA
GAGGCCTGAGACCTCTGCAGTGGGTAAAGGAAATGGGGGGGGGGGTAGGTACCTACTGTCCTCCAAGGAC
AGATAATGAGTGCAGGCATGCCATTCAGGAATCGAGCTGTCACACAGATTATTAAATGTTGCTTGGACTT
ATCACCCTGGGCAAGTTCCTTAGCATCTGGAACTTTCTATTTCCTGGCCTTAAAATAGGAACATAAACAG
TAGCTGCTTTTTAGAAGTATAAGGATTAATTACAAGAGATGGTGGCATGATCATCATTCTTATGACAAAT
GATCAGGGTGGTGAAGATAGTGGGTTCTTTAAAGGTTCTAGGATAAAACATATGGAGAGAGTGTCTCATT
ACGGGACCCAAGACACTCATTTCAAAAAGGCTTAACTGGTTACTTCTCACACACAGTATCACAAGGGTGT
AATATATGTAAGAGATATTCCATAGTCTATAGAAACAGAAGAACAGATACACAGTGGTCCCTTCCCACTC
ATGGCCGTCACCTGTGCCTGAAGCTCTCACGGTCCTCTTTGTTATACTCCAAGGCTGGCCCCCTGTTGGT
CCCACTGTTGCCTCCCATGACACCTGACCAGTAGTCAGGATTGCTTTCCAGGTCTCCAGACATAGGGAAC
TGCTTATTCCGCATCTGGTGGTAAGACTGGCGGAAATTGCTGTCATCATCATGCAGGGAGCTGAGTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 195239. Forward Primer - name:195239_F_exon_5330438I03Rik, sequence:ACATGTTTTACTTCACCCACCC; Reverse Primer - name:195239_N_SP6_exon_5330438I03Rik, sequence:GAACTCAGCTCCCTGCATGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8105 same embryo
 EurExpress:euxassay_016180 same experiment
 MGI:4822760 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS