EMAGE mouse gene expression spatial database

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EMAGE:8129

Lilra5 leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5 ( MGI:3647196)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016246. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016246_01	euxassay_016246_02	euxassay_016246_03	euxassay_016246_04

euxassay_016246_05	euxassay_016246_06	euxassay_016246_07	euxassay_016246_08	euxassay_016246_09

euxassay_016246_10	euxassay_016246_11	euxassay_016246_12	euxassay_016246_13	euxassay_016246_14

euxassay_016246_15	euxassay_016246_16	euxassay_016246_17	euxassay_016246_18	euxassay_016246_19

euxassay_016246_20	euxassay_016246_21	euxassay_016246_22	euxassay_016246_23	euxassay_016246_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8129_wholemount_strong.wlz

8129_wholemount_moderate.wlz

8129_wholemount_weak.wlz

8129_wholemount_possible.wlz

8129_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8129_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 09 weak expression: see section 16

facial vii ganglion

moderate

single cell

moderate expression: see section 04 05 20

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 06 07

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

ventral grey horn

moderate

single cell

moderate expression: see section 11 12 15 weak expression: see section 13 14

dorsal root ganglion

strong

single cell

strong expression: see section 08 09 moderate expression: see section 07 10 11 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45275
Entity Detected:	Lilra5, leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5 ( MGI:3647196)
Sequence:	sense strand is shown >T45275 GTTCTGGAAGAAAACATCTGGGACTACAATAGGGAACCATTTTCAGAGAACATTCAGGAAGAAAACATGA TTTGAGTGTAGGGAAACACCTGGGTACTTGGGTGGAAAATTCTTCTATGGCCAATCATCCTGTTATCACT TCACCTAGATGTATAACTTTGGCTGACAGTCTCCTCTGTCTCCCCACCTCCAATAATGAGGAGGGGATAA TCCTGTCTCATCAGTCAGGTTTGAGTCCTAATCTTGTGTATCTTCTGCCTGCCCCCCCCCCCACTTCCTT TATTATATGCTACCACATTCTCCACTTGCGTCATGAGCCTTCATTTTCTCTAATGAAAACAAAGAAGCTG GCTGAGATAGCAAAAATAATGCTCAAAAATCAGAGTCCAAAATGCAAAATGAGATCCTAATGTAAGTGAT GGCCCAGCATCTCTTGAACCCTCAGCTTTTAACACATGTTATTTATGTGATTTTCTTGAAATAGAAGACT TGCAATCAACAAACTTGCATTTGAAAGAATTCTCTGGTGGGGGATACTGCTGACATGGCTTTGAAAATAT CATTTTTTAAAAATAGTAATTAAAGAAGAGATCATGAATTTGGGAGGGAGTGTGGGAAGGCATAGGTGGA ATTGGAGAAAAAAAGAGGGGAATAAATGATGTAAATACAGTACTCATATAATAAATTCAGAAAAAATTAA GTAACCTTTCCTACTCATCCTCATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 203222. Forward Primer - name:203222_F_exon_E030017K20, sequence:GTTCTGGAAGAAAACATCTGGG; Reverse Primer - name:203222_N_SP6_exon_E030017K20, sequence:CATGAGGATGAGTAGGAAAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8132 same embryo
	EMAGE:8131 same embryo
	EMAGE:8128 same embryo
	EMAGE:8130 same embryo
	EMAGE:8133 same embryo
	EurExpress:euxassay_016246 same experiment
	MGI:4825924 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source