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EMAGE:8166

Nasp nuclear autoantigenic sperm protein (histone-binding) ( MGI:1355328)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8166 EMAGE:8166 EMAGE:8166 EMAGE:8166 EMAGE:8166
"Pseudo-wholemount" of euxassay_016401. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016401_01 euxassay_016401_02 euxassay_016401_03 euxassay_016401_04
EMAGE:8166 EMAGE:8166 EMAGE:8166 EMAGE:8166 EMAGE:8166
euxassay_016401_05 euxassay_016401_06 euxassay_016401_07 euxassay_016401_08 euxassay_016401_09
EMAGE:8166 EMAGE:8166 EMAGE:8166 EMAGE:8166 EMAGE:8166
euxassay_016401_10 euxassay_016401_11 euxassay_016401_12 euxassay_016401_13 euxassay_016401_14
EMAGE:8166 EMAGE:8166 EMAGE:8166
euxassay_016401_15 euxassay_016401_16 euxassay_016401_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8166Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8166_wholemount_strong.wlz
8166_wholemount_moderate.wlz
8166_wholemount_weak.wlz
8166_wholemount_possible.wlz
8166_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8166_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
weak weak
homogeneousweak expression: see section 01 02 03 04 05 07 09 10 11 12 13 14 15 16 17
rest of cerebellum marginal layer
weak weak
regionalweak expression: see section 03 04 11 12 13 14 15
rest of cerebellum ventricular layer
weak weak
regionalweak expression: see section 15
midbrain ventricular layer
weak weak
regionalweak expression: see section 06 07 08 09 10 11
metanephros
weak weak
regionalweak expression: see section 03 04 05 06 10 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63037
Entity Detected:Nasp, nuclear autoantigenic sperm protein (histone-binding) ( MGI:1355328)
Sequence:sense strand is shown

>T63037
GTTCTTATGGAACTGTCAGGGCAAGATGTTGAAGCATCACCAGTCGTGGCTGCAGAGGCCGGAGCTGAAG
TCTCTGAGAAGCCAGGGCAGGAGATTACAGTTATTCCCAACAATGGTCCAGTTGTTGGACAATCAACTGT
AGGAGATCAGACTCCTAGTGAACCACAGACTTCTGCAGAAAGACTGACAGAAACTAAAGATGGCTCAAGT
GTAGAGGAGGTCAAGGCAGAGCTGGTTCCTGAACAGGAGGAAGCTATGCTACCTGTAGAAGAGTCTGAGG
CAGCTGGAGATGGGGTTGAGACCAAGGTAGCCCAGAAGGCCACGGAGAAAGCACCTGAAGACAAATTTAA
GATAGCTGCTAATGAAGAGACACCAGAGAGAGACGAACAGATGAAAGAGGGTGAAGAAACTGAAGGCTCA
GAGGAAGAGGACAGAGAAAATGACAAGGCTGAAGAAACACCAAATGAATCAGTTCTTGAAAAAAAGTCTC
TTCAAGAAAATGAAGAGGAGGAGATAGGGAATTTGGAACTTGCCTGGGATATGCTGGATTTAGCCAAGAT
CATTTTTAAGAGGCAAGAAACAAAAGAAGCCCAGCTTTATGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141797. Forward Primer - name:141797_F_cDNA_Nasp, sequence:GTTCTTATGGAACTGTCAGGGC; Reverse Primer - name:141797_N_SP6_cDNA_Nasp, sequence:CAGCATAAAGCTGGGCTTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8164 same embryo
 EMAGE:8163 same embryo
 EMAGE:8161 same embryo
 EMAGE:8162 same embryo
 EMAGE:8165 same embryo
 EurExpress:euxassay_016401 same experiment
 MGI:4826599 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS