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EMAGE:8213

Uggt1 UDP-glucose glycoprotein glucosyltransferase 1 ( MGI:2443162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213
"Pseudo-wholemount" of euxassay_007335. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007335_01 euxassay_007335_02 euxassay_007335_03 euxassay_007335_04
EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213
euxassay_007335_05 euxassay_007335_06 euxassay_007335_07 euxassay_007335_08 euxassay_007335_09
EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213
euxassay_007335_10 euxassay_007335_11 euxassay_007335_12 euxassay_007335_13 euxassay_007335_14
EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213 EMAGE:8213
euxassay_007335_15 euxassay_007335_16 euxassay_007335_17 euxassay_007335_18 euxassay_007335_19
EMAGE:8213 EMAGE:8213
euxassay_007335_20 euxassay_007335_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8213Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8213_wholemount_strong.wlz
8213_wholemount_moderate.wlz
8213_wholemount_weak.wlz
8213_wholemount_possible.wlz
8213_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8213_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3004
Entity Detected:Uggt1, UDP-glucose glycoprotein glucosyltransferase 1 ( MGI:2443162)
Sequence:sense strand is shown

>T3004
TGGCCTCGAGGCCAGATTCGGCACGAGGTGGATTTGTACCTCAGAGGAAGACAAGTGGCTGGTCTTCTGG
GACTCTTCTTCTCACCAGAGGGAGAAATGCCGGCAGCAACTGCAAGAAGCCCAGTCCTTCGGTGCCCACA
GCCCAGAGCACACACTGCTGTGCTGGCCCAGAAGCTGGGCAGAAAGGTCACCATGTTCTTCCTTACCTCA
GTTTACCTGCAGCCCTTGCTGCAGTGCAGATGCCCACCCTGTACCAGGTCAGGCCGGCAGATGCTTCATC
CATGCCTCAAGGTCTACGCCAACATGCTTCTCACGTCTCTTCCCCTCCCCACCCCCCTTCCCAAGCACTA
GGCTTGTTGTTTAATGGAGCCCAACCAATAATCATGTGTCTTCAGGGTTGGCTCAGTGCAGTTGCTAAGC
AAATCAGTCTTCAGGAGAAACGGGGTGGTGTGGATAAGGCTGGAAGAAATGGCAGGTAGTGGAGAGTGTT
GGGGTGTTCGATGGAAGACAAAAACATGGGCCGTTCTCTTCAGTCTAGTACCCAGTGAACTGTCCAGGTA
ATTGTTTTG
Notes:The probe template was PCR amplified from IMAGE:1533341 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1533341 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8210 same embryo
 EMAGE:8212 same embryo
 EMAGE:8211 same embryo
 EurExpress:euxassay_007335 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS