EMAGE mouse gene expression spatial database

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EMAGE:8258

Zc3h7a zinc finger CCCH type containing 7 A ( MGI:2445044)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007309. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007309_01	euxassay_007309_02	euxassay_007309_03	euxassay_007309_04

euxassay_007309_05	euxassay_007309_06	euxassay_007309_07	euxassay_007309_08	euxassay_007309_09

euxassay_007309_10	euxassay_007309_11	euxassay_007309_12	euxassay_007309_13	euxassay_007309_14

euxassay_007309_15	euxassay_007309_16	euxassay_007309_17	euxassay_007309_18	euxassay_007309_19

euxassay_007309_20	euxassay_007309_21	euxassay_007309_22	euxassay_007309_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8258_wholemount_strong.wlz

8258_wholemount_moderate.wlz

8258_wholemount_weak.wlz

8258_wholemount_possible.wlz

8258_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8258_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 03

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 03

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 02

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 02 03

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 02

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 02 03

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 03

femur

moderate

regional

moderate expression: see section 02 03 17 22 weak expression: see section 19

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

mandible

moderate

regional

moderate expression: see section 08 09

meckel's cartilage

moderate

regional

moderate expression: see section 04 06 07 08 09 11 12 13 14 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 08 09 10 12 13 14 15 weak expression: see section 06 07

basioccipital bone

moderate

regional

moderate expression: see section 04 05 06

basisphenoid bone

moderate

regional

moderate expression: see section 12 13 14

exoccipital bone

moderate

regional

moderate expression: see section 03 04 05 06

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 23 weak expression: see section 21 22

vault of skull

moderate

regional

moderate expression: see section 01 02 03

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 19 23 weak expression: see section 21 22

viscerocranium

moderate

regional

Expression in the turbinate bone.

pelvic girdle skeleton

moderate

regional

moderate expression: see section 13

axial skeleton tail region

moderate

regional

moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1868
Entity Detected:	Zc3h7a, zinc finger CCCH type containing 7 A ( MGI:2445044)
Sequence:	sense strand is shown >T1868 TGGCCTCGAGGCCAGATTCGGCACGAGGACAATAAGTGCCTTGTCCACATTTTGAGAGAGACCACAGTAA AATATTCCAAAATACGTTCTTTTCATAAACAATGTCAATTTGATATTTGTCGACATGAAGTTCGTTATGG CTGCGTGAGGGAAGATGAGTGCTTCTATGCCCATAGTCTTATTGAACTGAAAGTCTGGATAATGCAAAGT GAAAGAGGTATCTCCCATGATGATATTGCTCAACAATCCAAACAATATTGGAGAAGTTTGCAAGCAAGCA TGCCGGGAGTGCAGGTCCTTGGTAATCAAATAATGCCTGGATTACTTAACATGGAGATAAAGTTTGTATG TGCTCAGTGTCTGAGAAATGGCCAAGTCATTGAACCAGACAGGAACAGAAAGTACTGCAGTGCCAAAGCA CAGCATTCGTGGACCAAAGAACGTCGTGCAATGAGAGCGATGTCTTTTGAACGTAAGAAGTGGATGAACA TCCGTCCTCTCCCCACAAAGAAACAAATGCCTTTACAGTTTGATCTATGCAACCATATT
Notes:	The probe template was PCR amplified from IMAGE:596354 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:596354 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8260 same embryo
	EMAGE:8259 same embryo
	EurExpress:euxassay_007309 same experiment
	MGI:4829267 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source