EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8260

Grem1 gremlin 1 ( MGI:1344337)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007295. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007295_01	euxassay_007295_02	euxassay_007295_03	euxassay_007295_04

euxassay_007295_05	euxassay_007295_06	euxassay_007295_07	euxassay_007295_08	euxassay_007295_09

euxassay_007295_10	euxassay_007295_11	euxassay_007295_12	euxassay_007295_13	euxassay_007295_14

euxassay_007295_15	euxassay_007295_16	euxassay_007295_17	euxassay_007295_18	euxassay_007295_19

euxassay_007295_20	euxassay_007295_21	euxassay_007295_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8260_wholemount_strong.wlz

8260_wholemount_moderate.wlz

8260_wholemount_weak.wlz

8260_wholemount_possible.wlz

8260_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8260_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20

humerus

moderate

regional

moderate expression: see section 01 02 03

femur

moderate

regional

moderate expression: see section 01 02 03 04 17 18 19 20

nasal septum

weak

regional

weak expression: see section 14

meckel's cartilage

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16 17 18 19 moderate expression: see section 03 04 05 06 11 12 20

axial skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

basioccipital bone

moderate

regional

moderate expression: see section 04 05 06 18 19 20

basisphenoid bone

moderate

regional

moderate expression: see section 11 12 13 14 15 16

exoccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22

frontal bone primordium

moderate

regional

moderate expression: see section 01

parietal bone

moderate

regional

moderate expression: see section 01 02

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 20

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

moderate

regional

moderate expression: see section 01 02 03

pelvic girdle skeleton

moderate

regional

moderate expression: see section 07 08 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1865
Entity Detected:	Grem1, gremlin 1 ( MGI:1344337)
Sequence:	sense strand is shown >T1865 TGGCCTCGAGGCCAGATTCGGCACGAGGAATTAATTCACTTGACCGCGGGTGTAAGTCTTTTGTCTTGTG AAGAACCTTCAGAATGTGGGGAGACACGTGGTGATGGCAAACGGGACAGAGGACTGACGCAGGAACGGTC AGGCTGAGGACCAGTCTGGGCCAGTGAAATTCAGTAGTGAGATGTCTAGAGTTTAAAAGTTGTTTCCCAA AACAATATTAGTCTTGTTTTTAGCAAAAGGGTTTTCCTGATATTTAAAAGAACCCAGACACACAGAGGAA AAATATAATCAGCAAAAAAACAAAACAAAACAAAATAACACAACAATAACAACAACAACAAACAAAAACC CAATTCTCTGTGCCAGCTTCTGTGACCTACTGATACTAGCTGTAACTGATACTAGCTGTTAAGGGTGAAA TGCTGACCACTCCTGTTTTAAGAACCAAGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:596225 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:596225 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8258 same embryo
	EMAGE:8259 same embryo
	EurExpress:euxassay_007295 same experiment
	MGI:4825220 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source