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EMAGE:8299

Paqr9 progestin and adipoQ receptor family member IX ( MGI:1922802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299
"Pseudo-wholemount" of euxassay_007688. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007688_01 euxassay_007688_02 euxassay_007688_03 euxassay_007688_04
EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299
euxassay_007688_05 euxassay_007688_06 euxassay_007688_07 euxassay_007688_08 euxassay_007688_09
EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299
euxassay_007688_10 euxassay_007688_11 euxassay_007688_12 euxassay_007688_13 euxassay_007688_14
EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299 EMAGE:8299
euxassay_007688_15 euxassay_007688_16 euxassay_007688_17 euxassay_007688_18 euxassay_007688_19
EMAGE:8299
euxassay_007688_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8299Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8299_wholemount_strong.wlz
8299_wholemount_moderate.wlz
8299_wholemount_weak.wlz
8299_wholemount_possible.wlz
8299_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8299_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 13 14 17 18 weak expression: see section 07 08 15 16 19
liver left lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11
liver right lobe
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36744
Entity Detected:Paqr9, progestin and adipoQ receptor family member IX ( MGI:1922802)
Sequence:sense strand is shown

>T36744
CTACCTGGACTACGCTTCCATCAGCTACTATGGCTTCGGCAGCACCGTGGCCTACTACTACTACCTGCTG
CCCAGCCTGAGCCTGCTGGACGCCAGGGTCATGACGCCGTATGTGCAGCAGCGCCTGGGCTGGCACGTGG
ACTGCACCCGCCTCATCGCGGTCTACCGCGCGCTCGTGCTGCCCGTGGCCTTCGTGCTGGCTGTGGCCTG
CACCGTGGCTTGCTGTAAGAGCCGCACCGACTGGTGCTCCTACCCGTTCGCCCTGCGCACCTTCGTCTTC
GTCATGCCGCTCAGCATGGCCTGCCCCATTATGCTAGAGAGCTGGCTCTTCGACCTGCGAGGCGAGAACC
CCACACTCTTCGTGCACTTCTACCGCCGCTACTTCTGGCTGGTGGTGGCCGCCTTCTTCAACGTGAGCAA
GATCCCTGAGCGCATCCAGCCGGGTCTCTTCGACATCATTGGCCACAGCCATCAGCTCTTCCACATCTTC
ACTTTCCTCAGCATCTATGACCAGGTGTACTACGTGGAAGAGGGCCTGCGCCAGTTCCTCCAGGCACCCC
CTGCCGCGCCCACCTTCTCGGGCACAGTGGGCTACATGCTGCTGCTGGTGGTTTGCCTGGGACTAGTCAT
CAGGAAGTTCCTGAACAGCACCGAATTCTGCAGTAAAAAGTGAGCCTCCACCCCCGGAGGAGGCTATGGT
TTGCCCACCGGTTTGCGTGGAGTTTCTGTTGTTGTTGTTGTTATTGTTGGTTTGTTTTCAAGTTTTGTTG
TTTCCTTCTTTGCTGGAAGAGGGTGCTGCAGGCCACTAGGGAAAGGTTCCCTGACAGTTGGGGAATCCCA
AGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96159. Forward Primer - name:096159_F_cDNA_Paqr9, sequence:CTACCTGGACTACGCTTCCATC; Reverse Primer - name:096159_N_SP6_cDNA_Paqr9, sequence:GACTTGGGATTCCCCAACTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8295 same embryo
 EMAGE:8296 same embryo
 EMAGE:8298 same embryo
 EMAGE:8294 same embryo
 EMAGE:8297 same embryo
 EurExpress:euxassay_007688 same experiment
 MGI:4827048 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS