EMAGE mouse gene expression spatial database

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EMAGE:8301

Nos1 nitric oxide synthase 1, neuronal ( MGI:97360)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007737. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007737_01	euxassay_007737_02	euxassay_007737_03	euxassay_007737_04

euxassay_007737_05	euxassay_007737_06	euxassay_007737_07	euxassay_007737_08	euxassay_007737_09

euxassay_007737_10	euxassay_007737_11	euxassay_007737_12	euxassay_007737_13	euxassay_007737_14

euxassay_007737_15	euxassay_007737_16	euxassay_007737_17	euxassay_007737_18	euxassay_007737_19

euxassay_007737_20	euxassay_007737_21	euxassay_007737_22	euxassay_007737_23	euxassay_007737_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8301_wholemount_strong.wlz

8301_wholemount_moderate.wlz

8301_wholemount_weak.wlz

8301_wholemount_possible.wlz

8301_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8301_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 11 13 14 15

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

moderate

regional

moderate expression: see section 04 10 11 12 13 14 20 21

medulla oblongata basal plate mantle layer

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

rest of cerebellum mantle layer

strong

spotted

strong expression: see section 08 09 10 11 12 13 14 15

pons mantle layer

strong

spotted

strong expression: see section 05 06 07 08 09 10 11 13 14 15 16 17 18 19

midbrain mantle layer

strong

spotted

strong expression: see section 09 12 13 14 15 16 17 18 moderate expression: see section 06 07 08 10 11

spinal cord mantle layer

strong

spotted

strong expression: see section 08 09 10 11 12 13 14 15

esophagus

strong

spotted

strong expression: see section 12 13

stomach

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09

midgut

strong

spotted

strong expression: see section 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36694
Entity Detected:	Nos1, nitric oxide synthase 1, neuronal ( MGI:97360)
Sequence:	sense strand is shown >T36694 GGCTAAGAAAGTCTTCAAGGCAGCCTGTGATGTGTTCTGTGTGGGGGATGACGTCAACATTGAGAAGGCG AACAATTCCCTCATTAGCAATGACCGCAGCTGGAAGAGGAATAAATTCCGCCTCACTTATGTGGCAGAAG CTCCAGAGCTGACCCAAGGTCTTTCCAATGTTCACAAAAAGCGAGTCTCAGCCGCCCGACTCCTCAGCCG CCAAAACCTGCAAAGTCCTAAATCCAGCCGATCGACCATCTTCGTGCGTCTCCACACCAACGGGAATCAG GAGTTGCAGTACCAGCCAGGGGACCACCTGGGTGTCTTCCCTGGCAACCATGAGGACCTCGTGAATGCAC TCATTGAACGGCTGGAGGACGCGCCGCCTGCCAACCACGTGGTGAAGGTGGAAATGCTGGAGGAAAGGAA CACGGCTCTGGGTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCACCCTGCACCATCTTCCAGGCC TTCAAGTACTACCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGCAGCAGTTCGCCTCCCTGGCCA CCAATGAGAAAGAGAAGCAGCGGCTGCTGGTCCTCAGCAAGGGGCTTCAGGAATATGAGGAATGGAAATG GGGCAAGAACCCCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATCCAGATGCCGGCCACACTTCTC CTCACACAGCTGTCGCTGCTACAACCTCGCTACTATTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98224. Forward Primer - name:098224_F_cDNA_Nos1, sequence:GGCTAAGAAAGTCTTCAAGGCA; Reverse Primer - name:098224_N_SP6_cDNA_Nos1, sequence:GGAATAGTAGCGAGGTTGTAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8304 same embryo
	EMAGE:8306 same embryo
	EMAGE:8303 same embryo
	EMAGE:8305 same embryo
	EMAGE:8302 same embryo
	EurExpress:euxassay_007737 same experiment
	MGI:4826751 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source