EMAGE mouse gene expression spatial database

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EMAGE:8320

Orc6 origin recognition complex, subunit 6 ( MGI:1929285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007673. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007673_01	euxassay_007673_02	euxassay_007673_03	euxassay_007673_04

euxassay_007673_05	euxassay_007673_06	euxassay_007673_07	euxassay_007673_08	euxassay_007673_09

euxassay_007673_10	euxassay_007673_11	euxassay_007673_12	euxassay_007673_13	euxassay_007673_14

euxassay_007673_15	euxassay_007673_16	euxassay_007673_17	euxassay_007673_18	euxassay_007673_19

euxassay_007673_20	euxassay_007673_21	euxassay_007673_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8320_wholemount_strong.wlz

8320_wholemount_moderate.wlz

8320_wholemount_weak.wlz

8320_wholemount_possible.wlz

8320_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8320_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 05 06 07 08 14 15 16 17

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 08 09 10 13 14 15

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

mandible

moderate

regional

moderate expression: see section 02 03 20 21

lower jaw incisor

moderate

regional

moderate expression: see section 09 10 13 14

lower jaw molar

moderate

regional

moderate expression: see section 05 06 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 09 10 13 14

upper jaw molar

moderate

regional

moderate expression: see section 05 06 17 18

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 18 weak expression: see section 12 13 14 15 16 17

liver right lobe

moderate

regional

moderate expression: see section 19 20 21 22

renal cortex

moderate

regional

moderate expression: see section 04 05 06 07 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36724
Entity Detected:	Orc6, origin recognition complex, subunit 6 ( MGI:1929285)
Sequence:	sense strand is shown >T36724 AGTCTGTCTGCACGTAGCTCAGAAACCAGCAATGCTGTCATATGCCTGGACCTTGCAGCTTCCTGTAGGA AGTGCCCCTTGGATAGAGCATATTTAATTAGGCTCTCTGGTTTGAACAAGATGATGTATCAGAGCTGTCT TAAATCTTTTGAGTGTTTACTAGGATTAAATTCAAATGTTGGAATAAGAGACCTAGCTGTACAGTTCAGC TGTACAGAAGCAGTGAACTTGGCTGCAGAGATACTGCAGAGCTACAAGTCTGGTCTTCCTGAAACACAGC GAGCAGATCTTGACTTGTCCAGGCCACTTTTCACCACTGCCGCACTACTCTCAGCATGCAAAATTCTAAA GATAAAGGTGGATAAAACCAAAATGATAACTACATCCGGTGTGAAAAAGGCAATACTTGATCGACTGTGT AAGCAGTTAGAAAAGATTGGGCAGCAGATTAACAGAGACTCTGCAGATTTAGCTAGACCAGCACTGAAGA GAAAGAAGCCAGAGTTTAGTCCAACACTGAAGAAAAAGGAGCCAGGACTTGAACCACCAGCAAAAGAAAT AGAAGTAATAGAAACCCTTCATAAACTACCTAAAGATGAAGATCTGACACAGGATTATGAAGAATGGAAA AGGAAGATTTTGGAAAATGCTGCCAAAGCTCAGACAGCCACAGCAGAGTGATTTCCATCTCACTGCAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100504. Forward Primer - name:100504_F_cDNA_Orc6l, sequence:AGTCTGTCTGCACGTAGCTCAG; Reverse Primer - name:100504_N_SP6_cDNA_Orc6l, sequence:GCCTGCAGTGAGATGGAAATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8321 same embryo
	EMAGE:8324 same embryo
	EMAGE:8319 same embryo
	EMAGE:8322 same embryo
	EMAGE:8323 same embryo
	EurExpress:euxassay_007673 same experiment
	MGI:4826984 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source