EMAGE mouse gene expression spatial database

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EMAGE:8327

Nts neurotensin ( MGI:1328351)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007634. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007634_01	euxassay_007634_02	euxassay_007634_03	euxassay_007634_04

euxassay_007634_05	euxassay_007634_06	euxassay_007634_07	euxassay_007634_08	euxassay_007634_09

euxassay_007634_10	euxassay_007634_11	euxassay_007634_12	euxassay_007634_13	euxassay_007634_14

euxassay_007634_15	euxassay_007634_16	euxassay_007634_17	euxassay_007634_18	euxassay_007634_19

euxassay_007634_20	euxassay_007634_21	euxassay_007634_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8327_wholemount_strong.wlz

8327_wholemount_moderate.wlz

8327_wholemount_weak.wlz

8327_wholemount_possible.wlz

8327_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8327_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

single cell

moderate expression: see section 11 14 15 16

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 14 moderate expression: see section 11 12 13 15

telencephalon mantle layer

strong

single cell

strong expression: see section 01 02 03 04 08 14 15 17 18 19 20 21 22 moderate expression: see section 16

olfactory cortex marginal layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

midbrain mantle layer

strong

spotted

strong expression: see section 07 08 10 11 15 16 17 moderate expression: see section 12

ventral grey horn

strong

single cell

strong expression: see section 10 11 12 13 14 15 16

heart ventricle

strong

single cell

strong expression: see section 06 07 08 09 10 11 12 13 14 15

lower jaw incisor

weak

regional

weak expression: see section 11 12 13

upper lip

strong

regional

strong expression: see section 06 07 08 10 11 12 13 14

upper jaw incisor

weak

regional

weak expression: see section 07 08 09 11 12 13

upper jaw molar

strong

regional

strong expression: see section 08 12

testis

moderate

regional

moderate expression: see section 06 07 17 18 weak expression: see section 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36711
Entity Detected:	Nts, neurotensin ( MGI:1328351)
Sequence:	sense strand is shown >T36711 AGAAGAAGATGTGAGAGCCCTGGAGGCAGATCTATTGACAAACATGCATACATCCAAGATCAGCAAAGCA AGTCCTCCGTCTTGGAAAATGACCTTGCTAAATGTTTGCAGCCTCATAAATAACGTGAACAGCCCGGCCG AGGAAGCAGGAGACATGCATGATGACGACCTTGTTGGCAAAAGGAAACTTCCCCTTGTTCTGGATGGTTT TAGCTTGGAAGCAATGCTGACCATCTTCCAGCTCCAGAAAATCTGCCGCAGCAGGGCCTTTCAACACTGG GAGATAATCCAGGAAGATATCCTTGATAACGTCAATGATAAAAACGAGAAGGAAGAAGTGATAAAGAGAA AAATCCCTTATATTCTGAAAAGGCAGCTGTATGAAAATAAACCCAGAAGGCCCTACATTCTCAAGAGGGG TTCCTACTACTACTGAGAAGCTAATTCTTGACCTGTGATTGTGATTGATTACCTGATATCTAGATATATA ATTATATGTGTGAATAAATGTGACAGAACTTGATTATTTCATCTTTTCCCACAATTGTGCTTTATTGGAT GTGATGTTTCTTGCATTCATGTAAATTGGATGCAATGTTTTCAAATAAATGTAGATCTTGAGCATGATGT GTTCTGTATAATTTTAATAGATAATAATCATAAAATATGTAGAACATTTTGTCAATTTGCAAGATAAGTT ATGGGTCACATGAACAGTCATGGCATTTAGCACGTTATAGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96304. Forward Primer - name:096304_F_cDNA_Nts, sequence:AGAAGAAGATGTGAGAGCCCTG; Reverse Primer - name:096304_N_SP6_cDNA_Nts, sequence:TCCTATAACGTGCTAAATGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8330 same embryo
	EMAGE:8326 same embryo
	EMAGE:8329 same embryo
	EMAGE:8325 same embryo
	EMAGE:8328 same embryo
	EurExpress:euxassay_007634 same experiment
	MGI:4826822 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source