EMAGE mouse gene expression spatial database

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EMAGE:8328

Ntrk3 neurotrophic tyrosine kinase, receptor, type 3 ( MGI:97385)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007633. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007633_01	euxassay_007633_02	euxassay_007633_03	euxassay_007633_04

euxassay_007633_05	euxassay_007633_06	euxassay_007633_07	euxassay_007633_08	euxassay_007633_09

euxassay_007633_10	euxassay_007633_11	euxassay_007633_12	euxassay_007633_13	euxassay_007633_14

euxassay_007633_15	euxassay_007633_16	euxassay_007633_17	euxassay_007633_18	euxassay_007633_19

euxassay_007633_20	euxassay_007633_21	euxassay_007633_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8328_wholemount_strong.wlz

8328_wholemount_moderate.wlz

8328_wholemount_weak.wlz

8328_wholemount_possible.wlz

8328_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8328_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 07 08 13 14

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 04 18 19 20 21 weak expression: see section 02 03 05 06 07 08 09 10 12 13 14 15 16 17

corpus striatum

moderate

regional

moderate expression: see section 04 weak expression: see section 03 16 17 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 11 12 13 14

trigeminal v ganglion

strong

single cell

strong expression: see section 03 04 05 06 07 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18

dorsal root ganglion

strong

single cell

strong expression: see section 07 08 09 10 11 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36710
Entity Detected:	Ntrk3, neurotrophic tyrosine kinase, receptor, type 3 ( MGI:97385)
Sequence:	sense strand is shown >T36710 TTGTCAAGTTCTATGGGGTGTGTGGTGATGGTGACCCACTCATCATGGTCTTTGAATACATGAAGCATGG AGACCTTAACAAGTTCCTCAGGGCCCATGGGCCAGATGCCATGATCCTCGTGGATGGACAGCCACGTCAG GCCAAGGGGGAGCTAGGGCTCTCTCAGATGCTCCACATCGCCAGTCAGATAGCCTCGGGCATGGTGTACC TGGCTTCCCAGCACTTTGTACACCGGGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95754. Forward Primer - name:095754_F_cDNA_Ntrk3, sequence:TTGTCAAGTTCTATGGGGTGTG; Reverse Primer - name:095754_N_SP6_cDNA_Ntrk3, sequence:GTCCCGGTGTACAAAGTGCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8330 same embryo
	EMAGE:8326 same embryo
	EMAGE:8329 same embryo
	EMAGE:8325 same embryo
	EMAGE:8327 same embryo
	EurExpress:euxassay_007633 same experiment
	MGI:4826821 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source