EMAGE mouse gene expression spatial database

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EMAGE:8339

Tapbp TAP binding protein ( MGI:1201689)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009813. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009813_01	euxassay_009813_02	euxassay_009813_03	euxassay_009813_04

euxassay_009813_05	euxassay_009813_06	euxassay_009813_07	euxassay_009813_08	euxassay_009813_09

euxassay_009813_10	euxassay_009813_11	euxassay_009813_12	euxassay_009813_13	euxassay_009813_14

euxassay_009813_15	euxassay_009813_16	euxassay_009813_17	euxassay_009813_18	euxassay_009813_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8339_wholemount_strong.wlz

8339_wholemount_moderate.wlz

8339_wholemount_weak.wlz

8339_wholemount_possible.wlz

8339_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8339_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 12

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 14 15 16 17 18 19

ventral grey horn

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30866
Entity Detected:	Tapbp, TAP binding protein ( MGI:1201689)
Sequence:	sense strand is shown >T30866 AACACCAGCACCCGTTGTGTGGGCTGCCCCAGGAGAGGCACCCCCAGAACTGCTCTGTCTTGCATCCCAC TTCTTCCCTTCGGAGGGTCTGGAGGTCAAGTGGGAGCTCAGAGGCGGCCCAGGAGGAAGTTCTAGAAAGG TTGAGGGGAAGACGTGGCTCTCCACCATCCGCCACCATTCCGATGGCTCTGTCAGCCAGTCTGGGCACCT GCAGCTACCTCCAGTCACTGCCAAGCAGCATGGAGTTCACTATGTCTGTCGGGTGTACCACTCTAGCCTG CCAGCATCGGGGCGCAGTGCTGACGTCACCCTGGAGGTGGCAGGCTTCTCAGGGCCCTCCATCGAGGACG GCATCGGCCTGTTCCTGTCTGCTTTTCTCCTCCTCGGACTCCTCAAGGTGCTAGGCTGGCTGGTAGCTGC CTACTGGACCATTCCTGAAGTCTCAAAGGAGAAGGCCACAGCTGCCAGCCTGACCATTCCCAGGAACTCA AAGAAGTCACAGTAAAGAAGTTCTCGTCTGTGGAAGCCACCCTCATCTCTGGCCCAGATGACTCCAGTAG CCCCTGCCCCAGAACAACAGCCTTCTTCTCTTCTTCTCCCTCCATTGAATAGCTCGGATTTTTTTTTTTT AAGGTTTTTTGTTTGTTTTAATCTTTCTTGTTTTGTTCTTGTTTTTAGACAAGGTCTCTCTATGTAGCCT TGGCTGGCTGTCCTGAACCCTCACCAAGCCAACCAGGCTGGCCTCGAAGTCCCAGAGATCCACCTGTCCC TCTGCCTCGGAGTGACAAACGGCACTGGACACCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4223922), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16760. Forward Primer - name:016760_F_IRAV24-27_C21_Tapbp, sequence:AACACCAGCACCCGTTGT; Reverse Primer - name:016760_R_SP6_IRAV24-27_C21_Tapbp, sequence:ATGGTGTCCAGTGCCGTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8342 same embryo
	EMAGE:8341 same embryo
	EMAGE:8340 same embryo
	EMAGE:8343 same embryo
	EurExpress:euxassay_009813 same experiment
	MGI:4828600 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source