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EMAGE:834

Igf2 insulin-like growth factor 2 ( MGI:96434)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:834
Fig 1E Lee et al, 1990 [PMID:1964408] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: A, anterior; P, posterior; D, distal; ve, visceral primitive endoderm; m, mesoderm (embryonic); ps, primitive streak; am, amnion; am, amniotic mesoderm; ch, chorion; al, allantois. The arrowhead indicates the approximate transition point between the columnar and visceral primitive endoderm, and the arrow denotes the approximate embryonic/extraembryonic boundary.
Expression Pattern Description
Spatial Annotation:
EMAGE:834EMAGE:834Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
834_voxel_strong_3D_1.wlz
834_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:834_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
amnion ectoderm
not detected not detected
On the basis of a rare unequivocal result showing exclusive localization of labelling in the mesodermal layer of the amnion, it is believed that the IGF-II gene is not expressed in the amniotic ectoderm
amnion mesoderm
detected detected
On the basis of a rare unequivocal result showing exclusive localization of labelling in the mesodermal layer of the amnion, it is believed that the IGF-II gene is not expressed in the amniotic ectoderm
allantois
detected detected
chorion ectoderm
detected detected
chorion mesoderm
detected detected
yolk sac endoderm
detected detected
yolk sac mesoderm
detected detected
embryo mesoderm
detected detected
regionalSignal was localized in the anterior-proximal embryonic mesoderm, and was continuous with labelling in extraembronic mesoderm. Labelling occupied approximately 1/4 of the distance between the extraembryonic/embryonic junction and the tip of the cylinder.
primitive streak
not detected not detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1344322
Entity Detected:Igf2, insulin-like growth factor 2 ( MGI:96434)
Sequence:sense strand is shown

>MGI:1344322
GGATCCCAGTGGGGAAGTCGATGTTGGTGCTTCTCATCTCTTTGGCCTTCGCCTTGTGCTGCATCGCTGC
TTACCGCCCCAGCGAGACTCTGTGCGGAGGGGAGCTTGTTGACACGCTTCAGTTTGTCTGTTCGGACCGC
GGCTTCTACTTCAGCAGGCCTTCAAGCCGTGCCAACCGTCGCAGCCGTGGCATCGTGGAAGAGTGCTGCT
TCCGCAGCTGCGACTTGGCCCTCCTGGAGACATACTGTGCCACCCCCGCCAAGTCCGAGAGGGACGTGTC
TACCTCTCAGGCCGTACTTCCGGACGACTTCCCCAGATACCCCGTGGGCAAGTTCTTCAAATTCGACACC
TGGAGACAGTCCGCGGGACGCCTGCGCAGAGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCGCATGC
TTGCCAAAGAGCTCGAAGCGTTCAGAGAGGCCAAGCGCCACCGTCCCCTGATCGTGTTACCACCCAAAGA
CCCCGCCCACGGGGGAGCCTCTTCGGAGATGTCCAGCAACCATCAGTGAACCAAAT
nt 1136 - nt 1681 of NM_031511.1
Notes:The Igf2 probe used in this study by Lee et al, 1990 [PMID:1964408] is denoted as the rat probe used by Stylianopoulou et al, 1988 [PMID:3246220] . The authors note that the rat and mouse sequences differ over this region by 10 out of 545 nt. Stylianopoulou et al in turn define the probe as "a 545-bp fragment from the rat IGF-II cDNA clone 27 (Soares et al, 1986 [PMID:2438416] ). This fragment extends between a BamHI site, located one nucleotide downstream from the ATG initiator, and an EcoRI linker present at the end of the fragment, three nucleotides downstream from the TGA terminator".
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Lee et al, 1990 [PMID:1964408] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1964408] Lee JE, Pintar J, Efstratiadis A 1990 Pattern of the insulin-like growth factor II gene expression during early mouse embryogenesis. Development (110):151-9
 [ PMID:3246220] Stylianopoulou F, Efstratiadis A, Herbert J, Pintar J 1988 Pattern of the insulin-like growth factor II gene expression during rat embryogenesis. Development (103):497-506
 [ doi:10.1016/0022-2836(86)90025-2] [ PMID:2438416] Soares MB, Turken A, Ishii D, Mills L, Episkopou V, Cotter S, Zeitlin S, Efstratiadis A 1986 Rat insulin-like growth factor II gene. A single gene with two promoters expressing a multitranscript family. J Mol Biol (192):737-52
Links:MGI:1344456 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI