EMAGE mouse gene expression spatial database

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EMAGE:8353

Gigyf1 GRB10 interacting GYF protein 1 ( MGI:1888677)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007703. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007703_01	euxassay_007703_02	euxassay_007703_03	euxassay_007703_04

euxassay_007703_05	euxassay_007703_06	euxassay_007703_07	euxassay_007703_08	euxassay_007703_09

euxassay_007703_10	euxassay_007703_11	euxassay_007703_12	euxassay_007703_13	euxassay_007703_14

euxassay_007703_15	euxassay_007703_16	euxassay_007703_17	euxassay_007703_18	euxassay_007703_19

euxassay_007703_20	euxassay_007703_21	euxassay_007703_22	euxassay_007703_23	euxassay_007703_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8353_wholemount_strong.wlz

8353_wholemount_moderate.wlz

8353_wholemount_weak.wlz

8353_wholemount_possible.wlz

8353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 03 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18 19 20

trigeminal v nerve

moderate

regional

moderate expression: see section 15

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 14 15

cervical ganglion

moderate

regional

moderate expression: see section 07 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 14

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36771
Entity Detected:	Gigyf1, GRB10 interacting GYF protein 1 ( MGI:1888677)
Sequence:	sense strand is shown >T36771 GTTGAGGGGACTGTCTCTGAGTCCTAGAATCAGTTCACCTCCTGGCCCACCTGGAGATCTAGAAGACGAA GAAGGCTTGAAGCACTTGCAGCAGGAGGCAGAGAAGCTGGTGGCCTCCCTGCAGGACAGCTCCCTGGAGG AGGAGCAGTTCACCGCTGCCATGCAAACCCAGGGCCTCCGCCACTCCACAGCTGCCACTGCCCTTCCCCT CAGCCATGGCGCTGCCCGAAAGTGGTTCTACAAGGACCCACAGGGGGAAATCCAAGGCCCTTTCACGACC CAGGAGATGGCTGAGTGGTTCCAGGCTGGCTACTTCTCCATGTCACTCTTAGTGAAGAGGGGCTGCGATG AGGGTTTCCAGCCTCTGGGAGAGGTGATCAAGATGTGGGGCCGTGTGCCCTTTGCCCCAGGGCCCTCGCC ACCCCCGCTGCTGGGCAACATGGATCAGGAGCGGCTGAAGAAGCAGCAGGAGCTGGCTGCGGCAGCCCTG TACCAGCAGCTGCAGCACCAGCACTTTCTGCAGCTGGTTGGCAGCCGCCAGCTCCCGCAGTGTACGACGC TCCGGGAAAAGGCAGCTATGGGGGACCTGACGCCGCCGCAGCAGCAGCAGCTCACTACGTTCCTGCAGCA GCTCCAGGCTCTCAAAACCCCCAGAGGTGGAGACCAGAACCTGCTCCCGACGATGAGCCGGTCCTTGTCG GTGCCAGATTCAGGCCCCCTCTGGGACTTACATACCTCAGCTTCATCACAGTCAGGTGGTGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66380. Forward Primer - name:066380_F_cDNA_Perq1, sequence:GTTGAGGGGACTGTCTCTGAGT; Reverse Primer - name:066380_N_SP6_cDNA_Perq1, sequence:CTCACCACCTGACTGTGATGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8352 same embryo
	EMAGE:8357 same embryo
	EMAGE:8354 same embryo
	EMAGE:8356 same embryo
	EMAGE:8355 same embryo
	EurExpress:euxassay_007703 same experiment
	MGI:4825053 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source