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EMAGE:8358

Pla2g3 phospholipase A2, group III ( MGI:2444945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358
"Pseudo-wholemount" of euxassay_009490. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009490_01 euxassay_009490_02 euxassay_009490_03 euxassay_009490_04
EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358
euxassay_009490_05 euxassay_009490_06 euxassay_009490_07 euxassay_009490_08 euxassay_009490_09
EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358
euxassay_009490_10 euxassay_009490_11 euxassay_009490_12 euxassay_009490_13 euxassay_009490_14
EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358 EMAGE:8358
euxassay_009490_15 euxassay_009490_16 euxassay_009490_17 euxassay_009490_18 euxassay_009490_19
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euxassay_009490_20 euxassay_009490_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8358Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8358_wholemount_strong.wlz
8358_wholemount_moderate.wlz
8358_wholemount_weak.wlz
8358_wholemount_possible.wlz
8358_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8358_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36801
Entity Detected:Pla2g3, phospholipase A2, group III ( MGI:2444945)
Sequence:sense strand is shown

>T36801
ATGTGGAGTCTCAAGGTGAGGTCCCTGACTAAGGGCTACAGTGTGGTGGCACATGCCTGCAATCTTAACA
CTTGAGGAGCTGAGGCAGGAGAATCATGAGTTTGAAGGAGCCTAGGACATAACAAGAAGTAATTTAGAAA
CAATAACAACAAAAGCTCCCACCCCATCCCTGTCCCAGAGTTCCCAAGAGGCCAGGCTTGGGTGGGGTCC
CATTGACTGATCACGTGACAGAGGTCCCTTGAGAAACATTCTTTGGCCTGCAGGGCTCCTTATCAAACCA
AGAAATCCAGAAACTGCAGTTTAGGATTCAAGGTTGGAAACATGGGACCTACCCTTGTCTGCTTCTGCCA
CTAGACTTGGGGCACAACGACATCTCAGAGGGAGCCTGAGGCTACCGCATAGTAAACCAGAGAGTTAGCG
TCTTACACTCTAGAAATGCCTCCTTTTACTTTGGCAACTAGGAACTTCAGTTCTAAACACAGTGACGTCA
TCCTGAGCATCCAGGTCTGTTTGGTTCCCCTGGTAGATGCTAACCCCCCAGGGAGGTTGAAGTTCCTTAT
GTAAAACAGTATATAGTATGTGCATGTAACTGTACATCCCTGTATATATTTTAATTCATTTCTAGATTAC
TTATAATAATGTAATGCAAATGACATGAAGGCTGTTGCTTTACTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75042. Forward Primer - name:075042_F_cDNA_Pla2g3, sequence:ATGTGGAGTCTCAAGGTGAGGT; Reverse Primer - name:075042_N_SP6_cDNA_Pla2g3, sequence:GCAGTAAAGCAACAGCCTTCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8360 same embryo
 EMAGE:8363 same embryo
 EMAGE:8359 same embryo
 EMAGE:8362 same embryo
 EMAGE:8361 same embryo
 EurExpress:euxassay_009490 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS