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EMAGE:837

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS14 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:837
Figure 6G of Norris et al., 2002 [PMID:12091315] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Ant - anterior; llpm - left lateral plate mesoderm; arrowhead - anterior extent of expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:837Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
837_wholemount_strong_3D_1.wlz
837_wholemount_moderate_3D_1.wlz
837_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:837_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
mesenchyme derived from lateral plate
detected detected
regionalExpression is in the left lateral plate mesoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1351202
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 probe used in this study by Norris et al., 2002 [PMID:12091315] is indicated as that previously described by Ryan et al., 1998 [PMID:9707115] ie. "Stage 22-24 chick limb bud and stage 26 Xenopus cDNA libraries were screened at reduced stringency with full-length mouse P-OTX. cDNAs were sequenced on both strands and the sequences deposited in Genbank (AF077092 and AF077767). Antisense probe for Xpitx2 spanned the entire ORF, cPitx2 the homeodomain and C terminus." It is unclear which of these probes was used by Norris et al., 2002 [PMID:12091315] .
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.0 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Norris et al., 2002 [PMID:12091315] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:12091315] Norris DP, Brennan J, Bikoff EK, Robertson EJ 2002 The Foxh1-dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. Development (129):3455-68
 [ doi:10.1038/29004] [ PMID:9707115] Ryan AK, Blumberg B, Rodriguez-Esteban C, Yonei-Tamura S, Tamura K, Tsukui T, de la Pena J, Sabbagh W, Greenwald J, Choe S, Norris DP, Robertson EJ, Evans RM, Rosenfeld MG, Izpisua Belmonte JC 1998 Pitx2 determines left-right asymmetry of internal organs in vertebrates. Nature (394):545-51
Links:MGI:2183531 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI