EMAGE mouse gene expression spatial database

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EMAGE:8384

Zbtb16 zinc finger and BTB domain containing 16 ( MGI:103222)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009508_01	euxassay_009508_02	euxassay_009508_03	euxassay_009508_04

euxassay_009508_05	euxassay_009508_06	euxassay_009508_07	euxassay_009508_08	euxassay_009508_09

euxassay_009508_10	euxassay_009508_11	euxassay_009508_12	euxassay_009508_13	euxassay_009508_14

euxassay_009508_15	euxassay_009508_16	euxassay_009508_17	euxassay_009508_18	euxassay_009508_19

euxassay_009508_20	euxassay_009508_21	euxassay_009508_22	euxassay_009508_23	euxassay_009508_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8384_wholemount_strong.wlz

8384_wholemount_moderate.wlz

8384_wholemount_weak.wlz

8384_wholemount_possible.wlz

8384_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8384_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 09 10 17 18 19 20

thymus primordium

moderate

spotted

moderate expression: see section 08 09 10 11 12 13

brain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 02 03 04 05 06 20 21 22 23 24

trigeminal v nerve

strong

regional

strong expression: see section 07 08 16 moderate expression: see section 09 15

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

vomeronasal organ

moderate

regional

moderate expression: see section 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36876
Entity Detected:	Zbtb16, zinc finger and BTB domain containing 16 ( MGI:103222)
Sequence:	sense strand is shown >T36876 GTCACTCCTGCAAGGAACTCTTCAGCCACCTGCAGGGCCTGAGGAGCCCACACTGGCAGGGGGTGGGCGA CACCCTGGGGTGGCTGAGGTGAAGATGGAAATGATGCAGGTAGATGAAGCCCCTGGCCAGGACAGCCCTG GGGCAGCTGAGTCTAGCATCTCAGGAGGGATGGGGGACAAGCTTGAAGAGAGGAGCAAAGAGGGGCCTGG GACCCCGACTCGAGGCAGTGTCATCACTAGTGCCAGAGAGCTGCATTATGGGAGAGAGGAGAGTGGTGAG CAGCTCTCGCCTCCTGTTGAAGCTGGCCAGGGACCCCCTGGGCGGCAGGAGCCCCTGGCACCCCCAGTGG AGAAGCATTTGGGTATCTACTCGGTGCTGCCCAACCACAAGGCCGATGCTGTGTTGAGCATGCCGTCTTC AGTGACGTCCGGTCTCCATGTGCAACCTGCCCTGGCAGTCTCCATGGACTTCAGCACCTACGGGGGTCTG CTGCCTCAGGGCTTCATCCAGAGGGAGCTGTTCAGCAAGCTGGGGGAGCTGGCTGTGGGCATGAAGGCTG AGAGCCGCCCTCTGGGGGAGCAGTGCAGCGTGTGTGGGGTCGAGCTTCCGGACAACGAGGCAGTGGAGCA GCACAGGAAACTGCACAGTGGGATGAAAACATACGGGTGTGAACTCTGCGGAAAAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81873. Forward Primer - name:081873_F_cDNA_Zbtb16, sequence:GTCACTCCTGCAAGGAACTCTT; Reverse Primer - name:081873_N_SP6_cDNA_Zbtb16, sequence:GTTTTCCGCAGAGTTCACACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8386 same embryo
	EMAGE:8381 same embryo
	EMAGE:8382 same embryo
	EMAGE:8383 same embryo
	EMAGE:8385 same embryo
	EurExpress:euxassay_009508 same experiment
	MGI:4829261 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source