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EMAGE:842

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:842
Figure 1D of Kitamura et al., 1999 [PMID:10572050] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: oft - outflow tract. The probe used detects the Pitx2c splice form of Pitx2.
Expression Pattern Description
Spatial Annotation:
EMAGE:842EMAGE:842Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
842_voxel_strong_3D_1.wlz
842_voxel_moderate_3D_1.wlz
842_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:842_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
outflow tract
detected detected
regionalExpression is detected in the left side of outflow tract.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1347569
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 probe used is this study by Kitamura et al., 1999 [PMID:10572050] was "prepared from cDNA equivalent to exonIII." The exact sequence is not specified but this probe detects the Pitx2c splice form (see Figs 1A,B).
Chemistry:RNA
Strand:antisense
Label:undefined
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:undefined
General Information
Authors:Kitamura et al., 1999 [PMID:10572050] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10572050] Kitamura K, Miura H, Miyagawa-Tomita S, Yanazawa M, Katoh-Fukui Y, Suzuki R, Ohuchi H, Suehiro A, Motegi Y, Nakahara Y, Kondo S, Yokoyama M 1999 Mouse Pitx2 deficiency leads to anomalies of the ventral body wall, heart, extra- and periocular mesoderm and right pulmonary isomerism. Development (126):5749-58
Links:MGI:1347773 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI